Publication: NFC-enabling smartphone-based portable amperometric immunosensor for hepatitis B virus detection
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Issued Date
2021
Resource Type
File Type
application/pdf
ISSN
9254005
Other identifier(s)
2-s2.0-85090715876
Rights
Srinakharinwirot University
Rights Holder(s)
Scopus
Bibliographic Citation
Sensors and Actuators, B: Chemical. Vol 326, (2021)
Suggested Citation
Teengam P., Siangproh W., Tontisirin S., Jiraseree-Amornkun A., Chuaypen N., Tangkijvanich P., Henry C.S., Ngamrojanavanich N., Chailapakul O. NFC-enabling smartphone-based portable amperometric immunosensor for hepatitis B virus detection. Sensors and Actuators, B: Chemical. Vol 326, (2021). doi:10.1016/j.snb.2020.128825 Retrieved from: https://hdl.handle.net/20.500.14740/5031
Abstract
A smartphone-controlled electrochemical sensor operated entirely via Near Field Communication (NFC) was used to create a simple label-free immunoassay for detecting Hepatitis B Virus (HBV). The completed system was composed of a card-sized electrochemical NFC tag sensor integrated with a smartphone and antibody-modified electrode sensor. Screen-printed graphene electrodes (SPGE) were modified with gold nanoparticles, increasing high sensitivity while β-cyclodextrin (β-CD) was electropolymerized on the surface to capture antibodies. The modification and immobilization processes were verified by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The NFC-based electrochemical immunosensor was then used to quantify Hepatitis B surface antigen (HBsAg) using amperometric detection by measuring the current from the (Fe(CN)6)3−/4− redox couple before and after addition of HBsAg. The linear calibration curve and limit of detection for HBsAg were found to be 10−200 μg/mL and 0.17 μg/mL, respectively. The sensor was then tested with chronic HBV infected serum samples and the system showed a good correlation with traditional immunoassays. This electrochemical immunosensor has the potential to be an alternative platform for portable, simple, sensitive and selective tools that can readily assess a variety of health indicators using a low-cost sensor system operated from a smartphone. © 2020 Elsevier B.V.
Subject(s)
Antibodies
Cyclic voltammetry
Electrochemical electrodes
Electrochemical impedance spectroscopy
Electrochemical sensors
Gold nanoparticles
Graphite electrodes
Immunosensors
Smartphones
Viruses
Amperometric detection
Amperometric immunosensors
Electrochemical immunosensors
Hepatitis B surface antigen
Immobilization process
Linear calibration curve
Screen-printed graphene electrodes
The near field communication (NFC)
Near field communication
Cyclic voltammetry
Electrochemical electrodes
Electrochemical impedance spectroscopy
Electrochemical sensors
Gold nanoparticles
Graphite electrodes
Immunosensors
Smartphones
Viruses
Amperometric detection
Amperometric immunosensors
Electrochemical immunosensors
Hepatitis B surface antigen
Immobilization process
Linear calibration curve
Screen-printed graphene electrodes
The near field communication (NFC)
Near field communication