Publication: Dual infections of IMNV and MrNV in cultivated Penaeus vannamei from Indonesia
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
448486
Other identifier(s)
2-s2.0-84869142891
Rights Holder(s)
Scopus
Bibliographic Citation
Aquaculture. Vol 372-375, No. (2013), p.70-73
Suggested Citation
Senapin S., Phiwsaiya K., Gangnonngiw W., Briggs M., Sithigorngul P., Flegel T.W. Dual infections of IMNV and MrNV in cultivated Penaeus vannamei from Indonesia. Aquaculture. Vol 372-375, No. (2013), p.70-73. doi:10.1016/j.aquaculture.2012.10.027 Retrieved from: https://hdl.handle.net/20.500.14740/6725
Abstract
Whitening of muscle tissue in farmed whiteleg shrimp Penaeus (Litopenaeus) vannamei can result from stress-induced muscle cramps and from viral infections caused by infectious myonecrosis virus (IMNV) or Penaeus vannamei nodavirus (PvNV). A similar viral-induced whitening of muscles can be caused in the river prawn Macrobrachium rosenbergii and in larvae of the penaeid shrimp Penaeus (Penaeus) monodon and Penaeus (Fenneropenaeus) indicus by a mixed infection with Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). Here we describe mixed infections of IMNV and MrNV in juvenile cultivated P. vannamei from Indonesia, detected by nested RT-PCR and immunohistochemical analysis. Muscle lesions in the dually-infected shrimp gave positive immunohistochemical reactions for both IMNV and MrNV, while connective tissue in the same samples gave positive reactions for MrNV only, indicating some differences in tissue specificity between the two viruses. Although it is not known whether the dual infections are more lethal to shrimp than single IMNV infections, this is possible since earlier work has shown that MrNV alone can increase mortality of P. vannamei under stress. © 2012 Elsevier B.V.
Subject(s)
Immunoassay
Infectious disease
Lesion
Mortality
Muscle
Polymerase chain reaction
Shrimp culture
Viral disease
Indonesia
Decapoda (Crustacea)
Fenneropenaeus
Fenneropenaeus indicus
Litopenaeus
Litopenaeus vannamei
Macrobrachium rosenbergii
Macrobrachium rosenbergii nodavirus
Metapenaeus bennettae
Nodaviridae
Penaeidae
Penaeus
Penaeus monodon
Infectious disease
Lesion
Mortality
Muscle
Polymerase chain reaction
Shrimp culture
Viral disease
Indonesia
Decapoda (Crustacea)
Fenneropenaeus
Fenneropenaeus indicus
Litopenaeus
Litopenaeus vannamei
Macrobrachium rosenbergii
Macrobrachium rosenbergii nodavirus
Metapenaeus bennettae
Nodaviridae
Penaeidae
Penaeus
Penaeus monodon