Publication:
Lupinifolin from Derris reticulata possesses bactericidal activity on Staphylococcus aureus by disrupting bacterial cell membrane

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

13403443

DOI

10.1007/s11418-016-1065-2

Other identifier(s)

2-s2.0-85007441143

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Natural Medicines. Vol 71, No.2 (2017), p.357-366

Suggested Citation

Yusook K., Weeranantanapan O., Hua Y., Kumkrai P., Chudapongse N. Lupinifolin from Derris reticulata possesses bactericidal activity on Staphylococcus aureus by disrupting bacterial cell membrane. Journal of Natural Medicines. Vol 71, No.2 (2017), p.357-366. doi:10.1007/s11418-016-1065-2 Retrieved from: https://hdl.handle.net/20.500.14740/4207

Title

Lupinifolin from Derris reticulata possesses bactericidal activity on Staphylococcus aureus by disrupting bacterial cell membrane

Author(s)

Yusook K.
 Weeranantanapan O.
 Hua Y.
 Kumkrai P.
 Chudapongse N.

Abstract

In this study, lupinifolin, a prenylated flavonoid, was isolated from Derris reticulata stem, identified by NMR spectra and confirmed with mass spectrometry. Lupinifolin was freshly prepared by solubilizing in 0.1 N NaOH and immediately diluted in Müller–Hinton broth for antibacterial testing. The data showed that Gram-positive bacteria were more susceptible to lupinifolin than Gram-negative bacteria. Of four strains of Gram-positive bacteria tested, Staphylococcus aureus was the most susceptible. Using the two-fold microdilution method, it was found that lupinifolin possessed antimicrobial activity against S. aureus with minimum inhibitory concentration and minimum bactericidal concentration of 8 and 16 µg/ml, respectively, which is less potent than ampicillin. However, from the time–effect relationship, it was shown that lupinifolin had faster onset than ampicillin. The faster onset of lupinifolin was confirmed by scanning electron microscopy. To investigate the mechanism of action of lupinifolin, transmission electron microscopy (TEM) was performed to observe the ultrastructure of S. aureus. The TEM images showed that lupinifolin ruptured the bacterial cell membrane and cell wall. Due to its fast onset, it is suggested that the action of lupinifolin is likely to be the direct disruption of the cell membrane. This hypothesis was substantiated by the data from flow cytometry using DiOC2 as an indicator. The result showed that the red/green ratio which indicated bacterial membrane integrity was significantly decreased, similar to the known protonophore carbonyl cyanide 3-chlorophenylhydrazone. It is concluded that lupinifolin inhibits the growth of S. aureus by damaging the bacterial cytoplasmic membrane. © 2016, The Japanese Society of Pharmacognosy and Springer Japan.

Subject(s)

Ampicillin
 Bactericide
 Derris reticulata extract
 Lupinifolin
 Plant extract
 Unclassified drug
 Antiinfective agent
 Flavonoid
 Lupinifolin
 Antibiotic sensitivity
 Article
 Bacterial cell wall
 Bactericidal activity
 Carbon nuclear magnetic resonance
 Controlled study
 Derris
 Derris reticulata
 Drug identification
 Drug isolation
 Drug mechanism
 Drug potency
 Drug screening
 Flow cytometry
 Mass spectrometry
 Minimum bactericidal concentration
 Minimum inhibitory concentration
 Nonhuman
 Plant stem
 Proton nuclear magnetic resonance
 Scanning electron microscopy
 Staphylococcus aureus
 Transmission electron microscopy
 Ultrastructure
 Cell membrane
 Chemistry
 Pathogenicity
 Staphylococcus aureus
 Anti-Bacterial Agents
 Cell Membrane
 Derris
 Flavonoids
 Plant Stems
 Staphylococcus aureus

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URI

https://hdl.handle.net/20.500.14740/4207

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Scopus: Year 1983-2021

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