Publication:
Over-expression and characterization of the alkalophilic, organic solvent-tolerant, and thermotolerant endo-l,4-ß-mannanase from Bacillus licheniformis isolate THCM 3.1

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Issued Date

2009

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Article

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application/pdf

ISSN

15131874

DOI

10.2306/scienceasia1513-1874.2009.35.017

Other identifier(s)

2-s2.0-64549152958

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Scopus

Bibliographic Citation

ScienceAsia. Vol 35, No.1 (2009), p.17-23

Suggested Citation

Kanjanavas F., Khawsak P., Pakpitcharoen A., Areekit S., Sriyaphai T., Pothivejkul K., Santiwatanakul S., Matsui K., Kajiwara T., Chansiri K. Over-expression and characterization of the alkalophilic, organic solvent-tolerant, and thermotolerant endo-l,4-ß-mannanase from Bacillus licheniformis isolate THCM 3.1. ScienceAsia. Vol 35, No.1 (2009), p.17-23. doi:10.2306/scienceasia1513-1874.2009.35.017 Retrieved from: https://hdl.handle.net/20.500.14740/7699

Title

Over-expression and characterization of the alkalophilic, organic solvent-tolerant, and thermotolerant endo-l,4-ß-mannanase from Bacillus licheniformis isolate THCM 3.1

Author(s)

Kanjanavas F.
 Khawsak P.
 Pakpitcharoen A.
 Areekit S.
 Sriyaphai T.
 Pothivejkul K.
 Santiwatanakul S.
 Matsui K.
 Kajiwara T.
 Chansiri K.

Abstract

Gene encoding for endo-1,4-ß-mannanase (EC 3.2.1.78) from Bacillus licheniformis THCM 3.1 was cloned and over-expressed in pET 100/D TOPO vector. The molecular weight of the purified enzyme was about 40 kDa. This enzyme had an optimum pH of 9 and an optimum temperature of 45 0C and retained up to 77% of its activity after incubation for 48 h. The activity of the enzyme was inhibited by 1OmM Of Pb2+, Ag+, Fe3+, Sn2+, Cu2+, and EDTA. Although partially inhibited, the enzyme retained much of its activity when the reaction solution was mixed with 15% (v/v) of the organic solvents acetone, toluene, benzene, dimethyl sulphoxide, 2-propanol, acetonitrile, or cyclohexane.

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https://hdl.handle.net/20.500.14740/7699

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Scopus: Year 1983-2021

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