Publication:
Immunocytochemical characterization of delta-opioid and Mu-opioid receptor protein in the bovine pineal gland

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

14226405

DOI

10.1159/000091718

Other identifier(s)

2-s2.0-33646447835

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Cells Tissues Organs. Vol 182, No.1 (2006), p.48-56

Suggested Citation

Phansuwan-Pujito P., Ebadi M., Govitrapong P. Immunocytochemical characterization of delta-opioid and Mu-opioid receptor protein in the bovine pineal gland. Cells Tissues Organs. Vol 182, No.1 (2006), p.48-56. doi:10.1159/000091718 Retrieved from: https://hdl.handle.net/20.500.14740/5865

Title

Immunocytochemical characterization of delta-opioid and Mu-opioid receptor protein in the bovine pineal gland

Author(s)

Phansuwan-Pujito P.
 Ebadi M.
 Govitrapong P.

Abstract

Opioidergic innervation has been identified in the mammalian pineal gland. Recently, opioid receptors in bovine pineal glands have been characterized; the activation of these receptors leads to the stimulation of melatonin synthesis. In this study, the precise localization of opioid receptors in bovine pineal glands was determined by an immunohistochemical technique using antibodies raised against delta-opioid and mu-opioid receptors. Immunoreactivity of these two receptors was present at a moderate level in pinealocytes. A double-labeling study has shown that delta-opioid receptors are localized predominantly with mu-opioid receptors in the same pinealocytes. These immunopositive pinealocytes are often located in a group; however, some of them are dispersed individually. In addition, both types of receptors were found in glial cells and processes. A small number of delta-receptor-immunoreactive nerve fibers were observed in the perivascular space and intraparenchyma of the pineal gland. Mu-opioid receptor immunoreactivity was found in a number of nerve fibers throughout the gland, and in terminal-like dots on pinealocytes. There was immunocolocalization between delta-opioid receptors or mu-opioid receptors and leu-enkephalin in some nerve fibers. The results of this study indicate that the modulatory effect of the opioid system on melatonin secretion in pineal glands might act via opioid receptors on pinealocytes, whereas receptors located on nerve fibers might modulate the release of opioid peptides. Copyright © 2006 S. Karger AG.

Subject(s)

Delta opiate receptor
 Leucine enkephalin
 Melatonin
 Mu opiate receptor
 Adolescent
 Animal tissue
 Article
 Cow
 Double labeling technique
 Extravascular space
 Glia cell
 Hormone release
 Hormone synthesis
 Immunocytochemistry
 Immunolocalization
 Nerve fiber transport
 Neuromodulation
 Nonhuman
 Parenchyma
 Perivascular space
 Pineal body
 Pinealocyte
 Priority journal
 Protein analysis
 Protein localization
 Protein secretion
 Receptor upregulation
 Technique
 Animals
 Cattle
 Immunohistochemistry
 Pineal Gland
 Receptors, Opioid, delta
 Receptors, Opioid, mu
 Staining and Labeling
 Animalia
 Bovinae
 Mammalia

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URI

https://hdl.handle.net/20.500.14740/5865

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Scopus: Year 1983-2021

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