Publication:
Mechanism of apoptosis induction associated with ERK1/2 upregulation via goniothalamin in melanoma cells

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

17920981

DOI

10.3892/etm.2018.5762

Other identifier(s)

2-s2.0-85041199338

Rights Holder(s)

Scopus

Bibliographic Citation

Experimental and Therapeutic Medicine. Vol 15, No.3 (2018), p.3052-3058

Suggested Citation

Tangchirakhaphan S., Innajak S., Nilwarangkoon S., Tanjapatkul N., Mahabusrakum W., Watanapokasin R. Mechanism of apoptosis induction associated with ERK1/2 upregulation via goniothalamin in melanoma cells. Experimental and Therapeutic Medicine. Vol 15, No.3 (2018), p.3052-3058. doi:10.3892/etm.2018.5762 Retrieved from: https://hdl.handle.net/20.500.14740/6247

Title

Mechanism of apoptosis induction associated with ERK1/2 upregulation via goniothalamin in melanoma cells

Author(s)

Tangchirakhaphan S.
 Innajak S.
 Nilwarangkoon S.
 Tanjapatkul N.
 Mahabusrakum W.
 Watanapokasin R.

Abstract

The present study aimed to investigate the effect of goniothalamin on apoptosis induction in the A375 melanoma cell line. Melanoma is a type of skin cancer with increased prevalence and no potential standard treatment. Goniothalamin is a plant, bioactive styrly-lactone, which has various bioactivities including anti-microbial, anti-inflammatory and anti-cancer. Apoptosis induction by goniothalamin has been studied in numerous cancer cell lines, however not in the melanoma cell line A375. The results of the MTT assay demonstrated that goniothalamin induced anti-proliferation in a dose dependent manner. Hoechst staining assay demonstrated that goniothalamin induced chromatin condensation and apoptotic bodies in A375 treated cells, and JC-1 staining revealed that goniothalamin induced mitochondrial membrane dysfunction in A375 cells. In addition, goniothalamin decreased the level of anti-apoptotic proteins myeloid cell leukemia 1, B cell lymphoma (Bcl)-2 and Bcl-extra large, whereas it increased the level of pro-apoptotic proteins, Bcl-2 Associated X, apoptosis regulator, t-BID and Bim in A375 treated cells. In addition, goniothalamin also increased active caspase-9, -7 and cleaved-poly (ADP-ribose) polymerase expression in A375 treated cells. Furthermore, phosphorylated (p)-pyruvate dehydrogenase kinase (PDK) 1 (Ser241) and p-RAC-alpha serine/threonine-protein kinase (Akt; Ser473) were decreased, however c-Jun and p-extracellular signal-regulated kinase (ERK)1/2 were increased upon goniothalamin treatment. These results suggest that goniothalamin has an effect, as anti-proliferation and apoptosis induction in A375 cells were associated with upregulated p-ERK1/2, c-Jun and downregulated p-PDK1 (Ser241), p-Akt (Ser473) in A375 cells. Therefore, goniothalamin may be a potential candidate for anti-cancer drug development for melanoma treatment. © 2018, Spandidos Publications. All rights reserved.

Subject(s)

Antineoplastic agent
 Caspase 7
 Caspase 9
 Goniothalamin
 Mitogen activated protein kinase 1
 Mitogen activated protein kinase 3
 Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
 Protein bcl 2
 Protein bcl xl
 Protein mcl 1
 Unclassified drug
 Analysis of variance
 Antiinflammatory activity
 Antimicrobial activity
 Antineoplastic activity
 Apoptosis
 Article
 Cell cycle assay
 Cell growth
 Cell viability assay
 Chromatin condensation
 Controlled study
 Cytotoxicity
 Fluorescence microscopy
 Human
 Human cell
 IC50
 Melanoma
 Mitochondrial membrane
 Mitochondrial membrane potential
 Morphological adaptation
 MTT assay
 Protein phosphorylation
 Receptor upregulation

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URI

https://hdl.handle.net/20.500.14740/6247

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Scopus: Year 1983-2021

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