Publication:
Phyllanthus emblica L. Enhances human umbilical vein endothelial wound healing and sprouting

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1741427X

DOI

10.1155/2013/720728

Other identifier(s)

2-s2.0-84877273108

Rights Holder(s)

Scopus

Bibliographic Citation

Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-

Suggested Citation

Chularojmontri L., Suwatronnakorn M., Wattanapitayakul S.K. Phyllanthus emblica L. Enhances human umbilical vein endothelial wound healing and sprouting. Evidence-based Complementary and Alternative Medicine. Vol 2013, No. (2013), p.-. doi:10.1155/2013/720728 Retrieved from: https://hdl.handle.net/20.500.14740/6660

Title

Phyllanthus emblica L. Enhances human umbilical vein endothelial wound healing and sprouting

Author(s)

Chularojmontri L.
 Suwatronnakorn M.
 Wattanapitayakul S.K.

Abstract

Endothelial dysfunction is the hallmark of impaired wound healing and increased risk of cardiovascular disease. Antioxidants from natural sources decrease oxidative stress and protect against cellular damage caused by reactive oxygen species (ROS). In this study, we examined the antioxidant constituents and capacity of Phyllanthus emblica L. (PE) fruit in freeze-dried power form. The pharmacological properties of PE were investigated using human umbilical vein endothelial cells (HUVECs) in the aspects of endothelial cell proliferation, nitric oxide (NO) production, wound healing, cell migration, in vitro angiogenesis, and VEGF gene expression. The ASC content of PE was 1.574% + 0.046% (w/w) as determined by HPLC and the total phenolic content was 36.1% ± 0.7% gallic acid equivalent when measured by Folin-Ciocalteu assay. The FRAP assay revealed a relatively high antioxidant capacity at 3,643 + 192.5 μmole/mg. PE at 0.1 to 10 μg/mL did not significantly influence endothelial cell proliferation, but at higher concentrations PE decreased cell survival to 62%. PE significantly promoted NO production, endothelial wound closure, endothelial sprouting, and VEGF mRNA expression. Therefore, PE is a candidate for antioxidant supplement that promotes endothelial function and restores wound healing competency. © 2013 Linda Chularojmontri et al.

Subject(s)

Antioxidant
 Emblica officinalis extract
 Gallic acid
 Messenger RNA
 Nitric oxide
 Phenol derivative
 Vasculotropin
 Wound healing promoting agent
 Angiogenesis
 Antioxidant activity
 Article
 Cell migration
 Cell proliferation
 Cell survival
 Cytotoxicity
 Emblica officinalis
 Fluorescence recovery after photobleaching
 Freeze drying
 Gene expression
 High performance liquid chromatography
 Human
 Human cell
 In vitro study
 Priority journal
 Umbilical vein endothelial cell
 Wound closure
 Wound healing

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URI

https://hdl.handle.net/20.500.14740/6660

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Scopus: Year 1983-2021

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