Publication:
Electrochemical detection of c-reactive protein based on anthraquinone-labeled antibody using a screen-printed graphene electrode

0
0

Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

399140

DOI

10.1016/j.talanta.2018.02.075

Other identifier(s)

2-s2.0-85042707817

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Talanta. Vol 183, (2018), p.311-319

Suggested Citation

Jampasa S., Siangproh W., Laocharoensuk R., Vilaivan T., Chailapakul O. Electrochemical detection of c-reactive protein based on anthraquinone-labeled antibody using a screen-printed graphene electrode. Talanta. Vol 183, (2018), p.311-319. doi:10.1016/j.talanta.2018.02.075 Retrieved from: https://hdl.handle.net/20.500.14740/4403

Title

Electrochemical detection of c-reactive protein based on anthraquinone-labeled antibody using a screen-printed graphene electrode

Author(s)

Jampasa S.
 Siangproh W.
 Laocharoensuk R.
 Vilaivan T.
 Chailapakul O.

Abstract

In this present work, a novel electrochemical immunosensor employing a screen-printed graphene electrode (SPGE) for a simple and highly sensitive determination of C-reactive protein (CRP) in a sandwich-type format was proposed. The sensor comprised of two CRP-specific antibodies: an unlabeled capture primary antibody (Anti-1°Ab) and an electrochemically detectable anthraquinone-labeled signaling secondary (AQ-2°Ab) antibody. The Anti-1°Ab was first covalently anchored onto an L-cysteine/gold-modified disposable SPGE (L-Cys/Au/SPGE) to create the anti-CRP surface. After binding of the CRP and the AQ-2°Ab, the electrochemical signal response was measured using differential pulse voltammetry (DPV). In the presence of CRP, the sensor exhibited a significant increase in the AQ current at AQ-2°Ab compared to the negative control. The CRP concentration was detected in the range of 0.01–150 µg/mL, and the limit of detection (LOD) (S/N = 3) and limit of quantitation (LOQ) (10 SD/Slope) were 1.5 ng/mL and 10 ng/mL, respectively. This sensor exhibited very high sensitivity in determining CRP and was successfully applied to detect CRP in certified human serum with satisfactory results. The developed sensor is suitable as an alternative method for determination of CRP and the same principle may be further applied to determine other clinically important target molecules. © 2018 Elsevier B.V.

Subject(s)

Amino acids
 Antibodies
 Aromatic compounds
 Electrochemical electrodes
 Electrochemical sensors
 Graphene
 Graphite electrodes
 Ketones
 Molecules
 Voltammetry
 Anthraquinone
 C-reactive proteins
 Differential pulse voltammetry
 ELectrochemical detection
 Electrochemical immunosensors
 Electrochemical signals
 Sandwich format
 Screen-printed graphene electrodes
 Chemical detection
 Anthraquinone derivative
 Antibody
 C reactive protein
 Graphite
 Chemistry
 Electrochemical analysis
 Electrode
 Genetic procedures
 Human
 Printing
 Anthraquinones
 Antibodies
 Biosensing Techniques
 C-Reactive Protein
 Electrochemical Techniques
 Electrodes
 Graphite
 Humans
 Printing

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4403

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By