Publication:
Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

14665026

DOI

10.1099/ijsem.0.003175

Other identifier(s)

2-s2.0-85061140267

Rights Holder(s)

Scopus

Bibliographic Citation

International Journal of Systematic and Evolutionary Microbiology. Vol 69, No.2 (2019), p.454-459

Suggested Citation

Niemhom N., Chutrakul C., Suriyachadkun C., Tadtong S., Thawai C. Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans. International Journal of Systematic and Evolutionary Microbiology. Vol 69, No.2 (2019), p.454-459. doi:10.1099/ijsem.0.003175 Retrieved from: https://hdl.handle.net/20.500.14740/5482

Title

Jiangella endophytica sp. nov., an endophytic actinomycete isolated from the rhizome of Kaempferia elegans

Author(s)

Niemhom N.
 Chutrakul C.
 Suriyachadkun C.
 Tadtong S.
 Thawai C.

Abstract

An endophytic actinobacterium, designated strain KE2-3 T , was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of LL-diaminopimelic acid in the cell peptidoglycan, MK-9(H 4 ) as the major menaquinone, iso-C 16: 0 , anteiso-C 15: 0 , iso-C 14: 0 and iso-C 15: 0 as the predominant fatty acids in cells, and the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in its membranes. Based on 16S rRNA gene sequence analysis, strain KE2-3 T was identified as a member of the genus Jiangella and showed the highest similarities to Jiangella muralis DSM 45357 T (99.3 %) followed by Jiangella alba DSM 45237 T (99.2 %), Jiangella alkaliphiliaDSM 45079 T (99.0 %), Jiangella gansuensis DSM 44835 T (98.8 %) and Jiangella mangrovi 3SM4-07 T (98.6 %). However, the draft genome sequence of strain KE2-3T exhibited low average nucleotide identity values to the reference strains (85.5-90.2 %), which were well below the 95-96%species circumscription threshold. The DNA G+C content of genomic DNA was 72.3 mol%. With the differences of physiological, biochemical and genotypic data, strain KE2-3 T could be discriminated from its closest neighbour. Thus, strain KE2-3 T should be recognised as a novel species of genus Jiangella, for which the name Jiangella endophytica sp. nov. is proposed. The type strain is KE2-3 T (=BCC 66359 T =NBRC 110004 T ). © 2019 IUMS.

Subject(s)

Cardiolipin
 Diaminopimelic acid
 Genomic DNA
 Mannoside
 Menaquinone
 Peptidoglycan
 Phosphatidylglycerol
 Phosphatidylinositol
 RNA 16S
 Bacterial DNA
 Diaminopimelic acid
 Farnoquinone
 Fatty acid
 Menaquinone
 Peptidoglycan
 Phospholipid
 RNA 16S
 Actinobacteria
 Article
 Bacterial strain
 Bacterium isolation
 DNA base composition
 DNA extraction
 Gene sequence
 Jiangella endophytica
 Kaempferia
 Kaempferia elegans
 Nonhuman
 Phylogeny
 Priority journal
 Rhizome
 Type strain
 Actinobacteria
 Analogs and derivatives
 Bacterium identification
 Cell wall
 Chemistry
 Classification
 DNA sequence
 Genetics
 Isolation and purification
 Microbiology
 Nucleic acid hybridization
 Nucleotide sequence
 Phylogeny
 Thailand
 Zingiberaceae
 Actinobacteria
 Bacterial Typing Techniques
 Base Composition
 Base Sequence
 Cell Wall
 Diaminopimelic Acid
 DNA, Bacterial
 Fatty Acids
 Nucleic Acid Hybridization
 Peptidoglycan
 Phospholipids
 Phylogeny
 Rhizome
 RNA, Ribosomal, 16S
 Sequence Analysis, DNA
 Thailand
 Vitamin K 2
 Zingiberaceae

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URI

https://hdl.handle.net/20.500.14740/5482

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Scopus: Year 1983-2021

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