Publication:
Survey of benign Theileria parasites of cattle and buffaloes in Thailand using allele-specific polymerase chain reaction of major piroplasm surface protein gene

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Issued Date

2003

Resource Type

Article

File Type

application/pdf

ISSN

9167250

DOI

10.1292/jvms.65.133

Other identifier(s)

2-s2.0-0038657797

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Veterinary Medical Science. Vol 65, No.1 (2003), p.133-135

Suggested Citation

Sarataphan N., Kakuda T., Chansiri K., Onuma M. Survey of benign Theileria parasites of cattle and buffaloes in Thailand using allele-specific polymerase chain reaction of major piroplasm surface protein gene. Journal of Veterinary Medical Science. Vol 65, No.1 (2003), p.133-135. doi:10.1292/jvms.65.133 Retrieved from: https://hdl.handle.net/20.500.14740/6638

Title

Survey of benign Theileria parasites of cattle and buffaloes in Thailand using allele-specific polymerase chain reaction of major piroplasm surface protein gene

Author(s)

Sarataphan N.
 Kakuda T.
 Chansiri K.
 Onuma M.

Abstract

During a year from 1999 to 2000, a total of 247 blood samples were collected from 214 cattle and 33 water buffaloes in 16 distinct geographical locations of Thailand and analyzed by allele-specific PCR amplification of major piroplasm surface protein (MPSP) genes of benign Theileria parasites. Four allelic MPSP gene types were determined namely C-type, I-type, B-type and Thai-type, which were originally designated from Japanese Theileria orientalis (Chitose, Ikeda), Australian T. buffeli (Warwick) and Thai T. sp. (Kamphaeng Saen), respectively. Only two allelic MPSP gene types were successively amplified from 204 (82.6%) blood samples. Among positive cases, 138 (67.6%) and 17 (8.3%) samples contained either Thai-type or C-type parasites, respectively, while 49 (24%) s amples contained both types. However, nucleotide sequences of MPSP genes of Thai T. sp. amplified by C-type specific primers revealed higher (96.3%) similarity to Indonesian T. sp. rather than (87.8% similarity) to Japanese T. orientalis (Chitose) designated as C-type.

Subject(s)

Bos taurus
 Bubalus
 Bubalus bubalis
 Theileria
 Theileria orientalis
 Membrane protein
 Protozoal protein
 Allele
 Animal
 Article
 Buffalo
 Cattle
 Classification
 Gene
 Genetic variability
 Genetics
 Heterozygote
 Isolation and purification
 Parasitology
 Polymerase chain reaction
 Thailand
 Theileria
 Alleles
 Animals
 Buffaloes
 Carrier State
 Cattle
 Genes, Protozoan
 Membrane Proteins
 Polymerase Chain Reaction
 Protozoan Proteins
 Thailand
 Theileria
 Variation (Genetics)

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URI

https://hdl.handle.net/20.500.14740/6638

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Scopus: Year 1983-2021

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