Publication:
Anti-angiogenic and anti-proliferative effects of benja-ummarit extract in rats with hepatocellular carcinoma

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

20499434

DOI

10.3892/br.2020.1272

Other identifier(s)

2-s2.0-85081572598

Rights Holder(s)

Scopus

Bibliographic Citation

Biomedical Reports. Vol 12, No.3 (2020), p.109-120

Suggested Citation

Kaewnoonual N., Itharat A., Pongsawat S., Nilbu-Nga C., Kerdput V., Pradidarcheep W. Anti-angiogenic and anti-proliferative effects of benja-ummarit extract in rats with hepatocellular carcinoma. Biomedical Reports. Vol 12, No.3 (2020), p.109-120. doi:10.3892/br.2020.1272 Retrieved from: https://hdl.handle.net/20.500.14740/4638

Title

Anti-angiogenic and anti-proliferative effects of benja-ummarit extract in rats with hepatocellular carcinoma

Author(s)

Kaewnoonual N.
 Itharat A.
 Pongsawat S.
 Nilbu-Nga C.
 Kerdput V.
 Pradidarcheep W.

Abstract

The herbal extract Benja-ummarit (BU) is a traditional Thai medicine with a putative cancer-suppressing effect. However, this effect has only been tested in vitro in human hepatocarcinoma cell lines. The present study determined the efficacy of a BU extract to treat hepatocellular carcinoma (HCC) in rats in vivo and established its anti-angiogenic and anti-proliferative properties. The BU extract was prepared in 95% ethanol and its composition determined using liquid chromatography-mass spectrometry. HCC was induced in Wistar rats by an injection of diethylnitrosamine (DEN), followed 2 weeks later by injections of thioacetamide (TAA) thrice weekly for 4 weeks. Following 2 months, the DEN-TAA-treated rats were divided into 6 groups that were treated orally for another 2 months with: i) No treatment; ii) vehicle; iii) 30 mg/kg sorafenib (SF); iv) 1 mg/kg BU; v) 10 mg/kg BU; or vi) 50 mg/kg BU. Liver samples were collected for gross morphological, histological, reverse transcription-quantitative PCR and western blot analyses, and serum samples were collected for liver function tests. The size and number of the cancer nodules were reduced ~10-fold in BU-treated HCC groups and ~14-fold in the SF-treated group compared with the HCC group. Furthermore, the serum parameters of liver damage were lower in BU-compared with SF-treated rats. These results indicate that while each of these formulations strongly reduce HCC expansion, BU extract results in less liver damage. Vascular endothelial growth factor expression was reduced significantly in the BU-and SF-treated HCC groups compared with the HCC group (P<0.05). BU extract antagonizes HCC growth in vivo potently through inhibiting tumor angiogenesis. BU, therefore, qualifies as a promising medical herb requiring further evaluation as a treatment of HCC. © 2020, Spandidos Publications. All rights reserved.

Subject(s)

2' para methoxycoumaroylaloeresin
 5 hydroxyaloin A
 Alanine aminotransferase
 Albumin
 Alkaloid
 Aloeresin B derivative
 Aloesin
 Aloin
 Angiogenesis inhibitor
 Antimitotic agent
 Benja ummarit extract
 Biochemical marker
 Dehydropipernoline
 Diethylnitrosamine
 Dihydrogambogic acid derivative
 Gambogic acid
 Pipercide
 Piperettine
 Pipericine
 Piperine
 Plant extract
 Sorafenib
 Thioacetamide
 Unclassified drug
 Vasculotropin
 Alanine aminotransferase blood level
 Albumin blood level
 Animal experiment
 Animal model
 Animal tissue
 Antiangiogenic activity
 Antiproliferative activity
 Article
 Blood sampling
 Body weight
 Cancer growth
 Chemical composition
 Comparative study
 Controlled study
 Down regulation
 Drug efficacy
 Herbal medicine
 Histopathology
 In vivo study
 Liquid chromatography-mass spectrometry
 Liver cell carcinoma
 Liver function test
 Liver histology
 Liver injury
 Liver tissue
 Liver weight
 Male
 Nonhuman
 Protein expression
 Rat
 Real time reverse transcription polymerase chain reaction
 Tumor vascularization
 Western blotting

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URI

https://hdl.handle.net/20.500.14740/4638

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Scopus: Year 1983-2021

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