Publication:
3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

23793694

DOI

10.1021/acssensors.8b01574

Other identifier(s)

2-s2.0-85065083634

Rights Holder(s)

Scopus

Bibliographic Citation

ACS Sensors. Vol 4, No.5 (2019), p.1211-1221

Suggested Citation

Yakoh A., Chaiyo S., Siangproh W., Chailapakul O. 3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications. ACS Sensors. Vol 4, No.5 (2019), p.1211-1221. doi:10.1021/acssensors.8b01574 Retrieved from: https://hdl.handle.net/20.500.14740/5331

Title

3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications

Author(s)

Yakoh A.
 Chaiyo S.
 Siangproh W.
 Chailapakul O.

Abstract

This article describes the device design and fabrication of two different configurations (flow-through and stopped-flow) of a sequential fluid delivery platform on a microfluidic paper-based device. The developed device is capable of storing and transporting reagents sequentially to the detection channel without the need for external power. The device comprises two components: An origami folding paper (oPAD) and a movable reagent-stored pad (rPAD). This 3D capillary-driven device eliminates the undesirable procedure of multiple-step reagent manipulation in a complex assay. To demonstrate the scope of this approach, the device is used for electrochemical detection of biological species. Using a flow-through configuration, a self-calibration plot plus real sample analysis using a single buffer introduction are established for ascorbic acid detection. We further broaden the effectiveness of the device to a complex assay using a stopped-flow configuration. Unlike other electrochemical paper-based sensors in which the user is required to cut off the device inlet or rest for the whole channel saturation before measurement, herein a stopped-flow device is carefully designed to exclude the disturbance from the convective mass transport. As a proof of concept, multiple procedures for electrode modification and voltammetric determination of serotonin are illustrated. In addition, the research includes an impedimetric label-free immunosensor for alpha;-fetoprotein using the modified stopped-flow device. The beneficial adVantages of simplicity, low sample volume (1 mu;L), and ability to perform a complex assay qualify this innovative device for use with diverse applications. © 2019 American Chemical Society.

Subject(s)

Ascorbic acid
 Chemical detection
 Microfluidics
 Paper
 ELectrochemical detection
 Electrochemical sensing
 Electrode modification
 Fluid delivery
 Label-free immunosensor
 Micro-fluidic devices
 Paper based devices
 Voltammetric determination
 Electrochemical sensors
 Alpha fetoprotein
 Gold
 Serotonin
 Chemistry
 Devices
 Electrochemistry
 Electrode
 Equipment design
 Immunoassay
 Lab on a chip
 Paper
 Alpha-Fetoproteins
 Electrochemistry
 Electrodes
 Equipment Design
 Gold
 Immunoassay
 Lab-On-A-Chip Devices
 Paper
 Serotonin

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URI

https://hdl.handle.net/20.500.14740/5331

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Scopus: Year 1983-2021

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