Publication: Biocompatibility study of modified injectable hyaluronic acid hydrogel with mannitol/BSA to alveolar bone cells
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
8853282
Other identifier(s)
2-s2.0-85095454840
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of Biomaterials Applications. (2020)
Suggested Citation
Areevijit K., Dhanesuan N., Luckanagul J.A., Rungsiyanont S. Biocompatibility study of modified injectable hyaluronic acid hydrogel with mannitol/BSA to alveolar bone cells. Journal of Biomaterials Applications. (2020). doi:10.1177/0885328220971746 Retrieved from: https://hdl.handle.net/20.500.14740/4769
Abstract
The quality and quantity of bone are crucial to the success of dental implant treatment. Recently, bone grafting materials have reached some limitations. This study aimed to evaluate the biocompatibility of novel drug delivery material, injectable methacrylated hyaluronic acid hydrogel incorporated with different ratios of mannitol and BSA (Man/BSA MeHA), to human alveolar bone cells. The three-dimensionally encapsulated cell culture was evaluated with the resazurin cell viability test, alkaline phosphatase activity assay, immunohistochemistry test for collagen type-I synthesis, and cell morphology. The results showed that the encapsulated cells were viable in all four ratios of Man/BSA MeHA hydrogel and the average metabolic rate was not less than the control group. The morphology test showed round shape cells at the upper portion of the hydrogel and fibroblast-like or polygonal shape at the lower portion of hydrogel next to the culture plate. All four groups could express enzyme alkaline phosphatase and collagen type-I. In conclusion, four ratios of Man/BSA MeHA hydrogel were biocompatible with primary human alveolar bone cells. © The Author(s) 2020.
Subject(s)
Biocompatibility
Bone
Cell culture
Collagen
Controlled drug delivery
Hyaluronic acid
Hydrogels
Morphology
Organic acids
Phosphatases
Polyols
ALkaline phosphatase
Alkaline phosphatase activity
Bone grafting materials
Drug delivery materials
Encapsulated cell
Hyaluronic acid hydrogels
Immunohistochemistry
Methacrylated hyaluronic acids
Cells
Bone
Cell culture
Collagen
Controlled drug delivery
Hyaluronic acid
Hydrogels
Morphology
Organic acids
Phosphatases
Polyols
ALkaline phosphatase
Alkaline phosphatase activity
Bone grafting materials
Drug delivery materials
Encapsulated cell
Hyaluronic acid hydrogels
Immunohistochemistry
Methacrylated hyaluronic acids
Cells