Publication:
Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

10117571

DOI

10.1159/000442164

Other identifier(s)

2-s2.0-84958106367

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Medical Principles and Practice. Vol 25, No.2 (2016), p.130-136

Suggested Citation

Aroonrerk N., Niyomtham N., Yingyoungnarongkul B.-E. Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts. Medical Principles and Practice. Vol 25, No.2 (2016), p.130-136. doi:10.1159/000442164 Retrieved from: https://hdl.handle.net/20.500.14740/5710

Title

Anti-Inflammation of N-Benzyl-4-Bromobenzamide in Lipopolysaccharide-Induced Human Gingival Fibroblasts

Author(s)

Aroonrerk N.
 Niyomtham N.
 Yingyoungnarongkul B.-E.

Abstract

Objective: To evaluate the effect of N-benzyl-4-bromobenzamide (NBBA) on lipopolysaccharide (LPS)-induced IL-6 and prostaglandin E2 (PGE2) production in human gingival fibroblasts (HGFs). Material and Methods: The benzamide compound was synthesized. The condition for IL-6 production of HGFs after induction with LPS was optimized. The HGFs were incubated with NBBA (10 μg/ml) for 30 min before LPS (1 μg/ml) was added. After 24 h of incubation time, the culture media were harvested and their IL-6 and PGE2 contents were determined using an enzyme-linked immunosorbent assay. Prednisolone (PDS) and NS-398 were used as positive controls. Statistical analysis of the IL-6 and PGE2 contents was performed using the ANOVA test followed by the Tukey multiple-comparisons test to compare replicate means. p < 0.001 was considered statistically significant. Results: The maximum IL-6 production was achieved when HGFs were exposed to 1 μg/ml of LPS for 24 h, which was inhibited by the IL-6 immunosuppressant PDS. The benzamide compound, NBBA, exhibited a potent anti-IL-6 activity with inhibition of 35.6 ± 0.5%, significantly different from in the LPS-induced HGFs (p < 0.001). In addition, it inhibited 75.6 ± 0.52% PGE2 production. Cell viability was not significantly affected by treatment with NBBA at a concentration <10 μg/ml (p < 0.001). Conclusions: NBBA exhibited an inhibitory effect on the production of IL-6 and PGE2 in LPS-induced HGFs. It could serve as a compound with inhibiting inflammatory activity in periodontal disease. © 2015 S. Karger AG, Basel.

Subject(s)

Antiinflammatory agent
 Benzamide derivative
 Interleukin 6
 Lipopolysaccharide
 N (2 cyclohexyloxy 4 nitrophenyl)methanesulfonamide
 N benzyl 4 bromobenzamide
 Prednisolone
 Prostaglandin E2
 Unclassified drug
 Antioxidant
 Interleukin 6
 Lipopolysaccharide
 N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
 Nitrobenzene derivative
 Prostaglandin E2
 Sulfonamide
 Analysis of variance
 Antiinflammatory activity
 Article
 Cell viability
 Controlled study
 Culture medium
 Cytokine production
 Enzyme linked immunosorbent assay
 Fibroblast
 Human
 Incubation time
 Orthodontics
 Periodontal disease
 Statistical analysis
 Biosynthesis
 Cell survival
 Drug effects
 Enzyme activation
 Fibroblast
 Metabolism
 Porphyromonas gingivalis
 Analysis of Variance
 Antioxidants
 Cell Survival
 Dinoprostone
 Enzyme Activation
 Fibroblasts
 Humans
 Interleukin-6
 Lipopolysaccharides
 Nitrobenzenes
 Porphyromonas gingivalis
 Sulfonamides

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URI

https://hdl.handle.net/20.500.14740/5710

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Scopus: Year 1983-2021

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