Publication: Bioactive metabolites from Spilanthes acmella Murr
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Issued Date
2009
Resource Type
File Type
application/pdf
ISSN
14203049
Other identifier(s)
2-s2.0-61449118746
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Molecules. Vol 14, No.2 (2009), p.850-867
Suggested Citation
Prachayasittikul S., Suphapong S., Worachartcheewan A., Lawung R., Ruchirawat S., Prachayasittikul V. Bioactive metabolites from Spilanthes acmella Murr. Molecules. Vol 14, No.2 (2009), p.850-867. doi:10.3390/molecules14020850 Retrieved from: https://hdl.handle.net/20.500.14740/7629
Abstract
Spilanthes acmella Murr. (Compositae) has been used as a traditional medicine for toothache, rheumatism and fever. Its extracts had been shown to exhibit vasorelaxant and antioxidant activities. Herein, its antimicrobial, antioxidant and cytotoxic activities were evaluated. Agar dilution method assays against 27 strains of microorganisms were performed. Results showed that fractions from the chloroform and methanol extracts inhibited the growth of many tested organisms, e.g. Corynebacterium diphtheriae NCTC 10356 with minimum inhibitory concentration (MIC) of 64-256 μg/mL and Bacillus subtilis ATCC 6633 with MIC of 128-256 μg/mL. The tested fractions all exhibited antioxidant properties in both DPPH and SOD assays. Potent radical scavenging activity was observed in the DPPH assay. No cytotoxic effects of the extracts against KB and HuCCA-1 cell lines were evident. Bioassay-guided isolation resulted in a diverse group of bioactive compounds such as phenolics [vanillic acid (2), trans-ferulic acid (5) and transisoferulic acid (6)], coumarin (scopoletin, 4) and triterpenoids like 3-acetylaleuritolic acid (1), β-sitostenone (3), stigmasterol and stigmasteryl-3-O-β-D- glucopyranosides, in addition to a mixture of stigmasteryl-and β-sitosteryl-3-O-β-D-glucopyranosides. The compounds 1-6 represent bioactive metabolites of S. acmella Murr, that were never previously reported. Our findings demonstrate for the first time the potential benefits of this medicinal plant as a rich source of high therapeutic value compounds for medicines, cosmetics, supplements and as a health food.
Subject(s)
Antiinfective agent
Antioxidant
Plant extract
Animal
Article
Asteraceae
Bacterium
Cell line
Chemical structure
Chemistry
Drug effect
Medicinal plant
Microbiological examination
Saccharomyces cerevisiae
Traditional medicine
Animals
Anti-Infective Agents
Antioxidants
Asteraceae
Bacteria
Cell Line
Medicine, Traditional
Microbial Sensitivity Tests
Molecular Structure
Plant Extracts
Plants, Medicinal
Saccharomyces cerevisiae
Antioxidant
Plant extract
Animal
Article
Asteraceae
Bacterium
Cell line
Chemical structure
Chemistry
Drug effect
Medicinal plant
Microbiological examination
Saccharomyces cerevisiae
Traditional medicine
Animals
Anti-Infective Agents
Antioxidants
Asteraceae
Bacteria
Cell Line
Medicine, Traditional
Microbial Sensitivity Tests
Molecular Structure
Plant Extracts
Plants, Medicinal
Saccharomyces cerevisiae