Publication:
Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility

0
0

Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

1253395

DOI

10.14456/sjst-psu.2020.28

Other identifier(s)

2-s2.0-85084370377

Rights Holder(s)

Scopus

Bibliographic Citation

Songklanakarin Journal of Science and Technology. Vol 42, No.1 (2020), p.213-221

Suggested Citation

Thongbamrer C., Niyomtham N., Chaiwut C., Posa C., Apiratikul N., Sangvichien E., Opanasopit P., Sakee U., Yingyongnarongkul B.-E., Radchatawedchakoon W. Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility. Songklanakarin Journal of Science and Technology. Vol 42, No.1 (2020), p.213-221. doi:10.14456/sjst-psu.2020.28 Retrieved from: https://hdl.handle.net/20.500.14740/4929

Title

Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility

Author(s)

Thongbamrer C.
 Niyomtham N.
 Chaiwut C.
 Posa C.
 Apiratikul N.
 Sangvichien E.
 Opanasopit P.
 Sakee U.
 Yingyongnarongkul B.-E.
 Radchatawedchakoon W.

Abstract

There were two objectives for this study. First, it focused on eight lipids having cholesterol as hydrophobic tails, carbamate linkers, and different polar headgroups. Second, it aimed to study the physicochemical properties of those eight lipids, including DNA binding, size, zeta potential, and the transfection efficiency. Cholesterol-1,2-diaminoethane and tetramethylguanidinium conjugated lipid showed the highest transfection efficacy into human embryonic kidney cells cells. The optimal formulation of this lipid was found to be 1:1 (weight/weight) for cationic lipid/DOPE, and 1:20 for DNA/liposome. GFP expression experiments further revealed that the liposome exhibited higher transfection efficiency under a 10−40% serum condition than Lipofectamine™ 2000. © 2020, Prince of Songkla University. All rights reserved.

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4929

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By