Publication:
Melatonin attenuates the high-fat diet and streptozotocin-induced reduction in rat hippocampal neurogenesis

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

1970186

DOI

10.1016/j.neuint.2016.09.006

Other identifier(s)

2-s2.0-84988487530

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Neurochemistry International. Vol 100, (2016), p.97-109

Suggested Citation

Wongchitrat P., Lansubsakul N., Kamsrijai U., Sae-Ung K., Mukda S., Govitrapong P. Melatonin attenuates the high-fat diet and streptozotocin-induced reduction in rat hippocampal neurogenesis. Neurochemistry International. Vol 100, (2016), p.97-109. doi:10.1016/j.neuint.2016.09.006 Retrieved from: https://hdl.handle.net/20.500.14740/5060

Title

Melatonin attenuates the high-fat diet and streptozotocin-induced reduction in rat hippocampal neurogenesis

Author(s)

Wongchitrat P.
 Lansubsakul N.
 Kamsrijai U.
 Sae-Ung K.
 Mukda S.
 Govitrapong P.

Abstract

A deviant level of melatonin in blood circulation has been associated with the development of diabetes and with learning and memory deficiencies. Melatonin might have an important function in diabetes control; however, the mechanism of melatonin in diabetes remains unknown. The present study aimed to investigate the hyperglycemic condition induced by high-fat diet (HFD) feeding and streptozotocin (STZ) injection and to examine the effect of melatonin on adult hippocampal functions. HFD-fed and STZ-treated rats significantly increased blood glucose level. The present study showed that HFD-fed and STZ-treated rats significantly impaired memory in the Morris Water Maze task, reduced neurogenesis in the hippocampus shown by a reduction in nestin, doublecortin (DCX) and β-III tubulin immunoreactivities, reduced axon terminal markers, synaptophysin, reduced dendritic marker including postsynaptic density 95 (PSD-95) and the glutamate receptor subunit NR2A. Moreover, a significant downregulation of melatonin receptor, insulin receptor-β (IR-β) and both p-IR-β and phosphorylated extracellular signal-regulated kinase (p-ERK) occurred in HFD-fed and STZ-treated rats, while the level of glial fibrillary acidic protein (GFAP) increased. Treatment of melatonin, rats had shorter escape latencies and remained in the target quadrant longer compared to the HFD-fed and STZ-treated rats. Melatonin attenuated the reduction of neurogenesis, synaptogenesis and the induction of astrogliosis. Moreover, melatonin countered the reduction of melatonin receptor, insulin receptor and downstream signaling pathway for insulin. Our data suggested that the dysfunction of insulin signaling pathway occurred in the diabetes may provide a convergent mechanism of hippocampal impaired neurogenesis and synaptogenesis lead to impair memory while melatonin reverses these effects, suggesting that melatonin may reduce the pathogenesis of diabetes. © 2016 Elsevier Ltd

Subject(s)

Beta tubulin
 Doublecortin
 Glial fibrillary acidic protein
 Glucose
 Insulin
 Insulin receptor
 Melatonin
 Melatonin receptor
 Mitogen activated protein kinase
 N methyl dextro aspartic acid receptor 2A
 Nestin
 Postsynaptic density protein 95
 Streptozocin
 Synaptophysin
 Glutamate receptor
 Melatonin
 Streptozocin
 Synaptophysin
 Animal experiment
 Article
 Astrocytosis
 Body weight
 Controlled study
 Diabetes mellitus
 Down regulation
 Enzyme phosphorylation
 Glucose blood level
 Hippocampus
 Hyperglycemia
 Immunoreactivity
 Lipid diet
 Male
 Memory disorder
 Nerve cell plasticity
 Nervous system development
 Nonhuman
 Priority journal
 Rat
 Signal transduction
 Synaptogenesis
 Animal
 Drug effects
 Hippocampus
 Hyperglycemia
 Memory
 Metabolism
 Nervous system development
 Wistar rat
 Animals
 Diet, High-Fat
 Hippocampus
 Hyperglycemia
 Male
 Melatonin
 Memory
 Memory Disorders
 Neurogenesis
 Rats, Wistar
 Receptors, Glutamate
 Streptozocin
 Synaptophysin

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URI

https://hdl.handle.net/20.500.14740/5060

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Scopus: Year 1983-2021

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