Publication: Potential α-glucosidase inhibitors from cultures of Biscogniauxia capnodes SWUF15-40 fungus
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Issued Date
2025-05-01
Resource Type
ISSN
13403443
eISSN
18610293
Scopus ID
2-s2.0-85218777700
Pubmed ID
40009286
Journal Title
Journal of Natural Medicines
Volume
79
Issue
3
Start Page
488
End Page
498
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Natural Medicines Vol.79 No.3 (2025) , 488-498
Suggested Citation
Churat A., Katrun P., Laohpongspaisan C., Mongkolthanaruk W., Champasri C., Moontragoon P., Suwannasai N., Sangvichien E., Poonsukkho P., Mccloskey S. Potential α-glucosidase inhibitors from cultures of Biscogniauxia capnodes SWUF15-40 fungus. Journal of Natural Medicines Vol.79 No.3 (2025) , 488-498. 498. doi:10.1007/s11418-025-01876-9 Retrieved from: https://hdl.handle.net/20.500.14740/20773
Corresponding Author(s)
Other Contributor(s)
Abstract
The search for a potent α-glucosidase inhibitor from the fungus Biscogniauxia capnodes SWUF15-40 yielded eighteen compounds. A comprehensive analysis from NMR and MS data revealed three new α-pyrones, biscogniapyrones A–C (1–3), two new isocoumarins (5 and 6), and thirteen known compounds. The configurations were assigned from calculated 13C NMR chemical shifts and ECD spectra, together with 1H NMR analysis of Mosher esters. Several compounds exhibited effective inhibitory activity against α-glucosidase with IC50 values in the range of 0.041–0.257 mM, which are lower than the positive control, acarbose (IC50 0.713 mM). The proposed non-competitive mode of inhibition was deduced from Lineweaver–Burk plots together with Km and Vmax values. In silico dockings of the strongest inhibitor, compound 3 were studied. Three out of the five determined allosteric sites of the enzyme model were favorable, with closed free binding energies of roughly − 4.00 kcal/mol. The binding interactions observed between 3 and amino acids in the pocket sites were hydrogen bonding and hydrophobic interactions. These findings, therefore, provide opportunities for drug development processes to be carried out.