Publication: A piezoelectric-based immunosensor for high density lipoprotein particle measurement
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Issued Date
2014
Resource Type
File Type
application/pdf
ISSN
32654
Other identifier(s)
2-s2.0-84906092923
Rights Holder(s)
Scopus
Bibliographic Citation
Analyst. Vol 139, No.18 (2014), p.4586-4592
Suggested Citation
Chunta S., Suk-Anake J., Chansiri K., Promptmas C. A piezoelectric-based immunosensor for high density lipoprotein particle measurement. Analyst. Vol 139, No.18 (2014), p.4586-4592. doi:10.1039/c4an00601a Retrieved from: https://hdl.handle.net/20.500.14740/6485
Author(s)
Abstract
A piezoelectric-based immunosensor was developed for high density lipoprotein particle (HDL-P) measurement. Monoclonal anti-human apolipoprotein A1 antibody was used as a specific binding molecule for the major apolipoprotein of HDL-P. This sensing element was fabricated by immobilizing the anti-human apolipoprotein A1 on a 12 MHz AT-cut quartz crystal via a 3-mercaptopropionic acid (MPA) self-assembled monolayer. The frequency shift from the mass change of the antigen-antibody binding refers to the amount of HDL-P. The optimal antibody immobilization was performed to achieve the maximum potential of the antibody. The appropriate quantity and immobilization time of the antibody were 0.1 mg ml−1 and 90 minutes, respectively. The immobilized antibody in the HDL-P immunosensor accomplished perfect binding with HDL-P within 60 minutes. The dose-response curve for HDL-P showed a linear response from 0.21 to 2.50 mg protein per ml equivalent to 0.40 × 1010 to 3.65 × 1010 particles per μl without significant interference from other lipoproteins. The intra- and inter-assay imprecision (CV) were 7.8 and 18.5%, respectively. The analytical accuracy of this measurement was 96.29-96.31%. The HDL-P concentration obtained from the sensor revealed a 2.05 mg protein per ml with 0.26 mg protein per ml of expanded uncertainty at the 95% confidence level. This immunosensor gave an assay result which correlated with the homogeneous enzymatic colorimetric assay (R2 = 0.902). © 2014 the Partner Organisations.