Publication:
Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

16159306

DOI

10.1002/jssc.201600475

Other identifier(s)

2-s2.0-84988380509

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Separation Science. Vol 39, No.18 (2016), p.3521-3527

Suggested Citation

Chaloemsuwiwattanakan T., Sangcakul A., Kitiyakara C., Nacapricha D., Wilairat P., Chaisuwan P. Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column. Journal of Separation Science. Vol 39, No.18 (2016), p.3521-3527. doi:10.1002/jssc.201600475 Retrieved from: https://hdl.handle.net/20.500.14740/5253

Title

Simple and fast analysis of iohexol in human serums using micro-hydrophilic interaction liquid chromatography with monolithic column

Author(s)

Chaloemsuwiwattanakan T.
 Sangcakul A.
 Kitiyakara C.
 Nacapricha D.
 Wilairat P.
 Chaisuwan P.

Abstract

A simple and rapid method based on micro-liquid chromatography using a synthetic monolithic capillary column was developed for determination of iohexol in human serums, a marker to evaluate the glomerular filtration rate. A hydrophilic methacrylic acid-ethylene dimethacrylate monolith provided excellent selectivity and efficiency for iohexol with separation time of 3 min using a mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5/methanol. Four serum protein removal, methods using perchloric acid, 50% acetonitrile, 0.1 M zinc sulfate, and centrifuge membrane filter were examined. The method of zinc sulfate was chosen due to its simplicity, compatibility with the mobile phase system, nontoxicity, and low cost. Interday calibration curves were conducted over iohexol concentrations range of 2–500 mg/L (R2 = 0.9997 ± 0.0001) with detection limit of 0.44 mg/L. Intra- and interday precisions for peak area and retention time were less than 2.8 and 1.4%, respectively. The method was successfully applied to serum samples with percent recoveries from 102 to 104. The method was applied to monitor released iohexol from healthy subject. Compared with the commercially available reversed-phase high-performance liquid chromatography method, the presented method provided simpler chromatogram, faster separation with higher separation efficiency and much lower sample and solvent consumption. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Subject(s)

Body fluids
 Chromatographic analysis
 Chromatography
 Column chromatography
 Efficiency
 Ethylene
 Filtration
 High performance liquid chromatography
 Hydrophilicity
 Liquids
 Separation
 Zinc
 Ethylene dimethacrylate
 Glomerular filtration rate
 Hydrophilic interaction liquid chromatographies
 Iohexol
 Micro-liquid chromatography
 Monolithic capillary column
 Reversed phase high performance liquid chromatography
 Separation efficiency
 Liquid chromatography
 Acetonitrile
 Ethylene glycol dimethacrylate
 Iohexol
 Methacrylic acid
 Methanol
 Perchloric acid
 Zinc sulfate
 Article
 Calibration
 Controlled study
 Drug blood level
 Drug determination
 Drug structure
 Glomerulus filtration rate
 Human
 Hydrophilic interaction chromatography
 Limit of detection
 Membrane filter
 Normal human
 PH
 Priority journal
 Retention time
 Reversed phase high performance liquid chromatography
 Serum

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URI

https://hdl.handle.net/20.500.14740/5253

Collections

Scopus: Year 1983-2021

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