Publication: Effects of oxyresveratrol and its derivatives on cultured P19-derived neurons
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Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
15965996
Other identifier(s)
2-s2.0-85007138029
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Tropical Journal of Pharmaceutical Research. Vol 15, No.12 (2016), p.2619-2628
Suggested Citation
Tadtong S., Chatsumpun N., Sritularak B., Jongbunprasert V., Ploypradith P., Likhitwitayawuid K. Effects of oxyresveratrol and its derivatives on cultured P19-derived neurons. Tropical Journal of Pharmaceutical Research. Vol 15, No.12 (2016), p.2619-2628. doi:10.4314/tjpr.v15i12.12 Retrieved from: https://hdl.handle.net/20.500.14740/4910
Abstract
Purpose: To modify the structure of oxyresveratrol and evaluate the obtained derivatives for effects on neuronal cells. Methods: Electron-withdrawing groups were selectively introduced to the aromatic ring of the core stilbene structure. Oxyresveratrol and derivatives were then evaluated for their ability to enhance the survival of P19 derived neuronal cells by XTT method, in comparison with the widely known antioxidants, Trolox and ascorbic acid. Phase-contrast microscopic images of the neurons under various conditions were also taken and analyzed. Results: Oxyresveratrol, at a very low concentration (1 ng/mL), enhanced the survival of neurons in both normal and serum-deprivation conditions. Higher activity was observed for the 5-formylated and t 5-carboxylated products. The potencies of these polyoxygenated stilbenes were far greater than those of Trolox and ascorbic acid. These observations were supported by results from the examination of the phase-contrast micrographs of the neuronal cells. Conclusion: Oxyresveratrol and some derivatives prepared in this study demonstrate significant cell protective activity and may be of therapeutic value, but further investigations in animals are required to verify their neuroprotective potentials. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
Subject(s)
2,3′,4,5′ tetraisopropoxy stilbene
2′ formyl 2,3′,4,5′ tetrahydroxystilbene
2′ formyl 2,3′,4,5′ tetraisopropoxy stilbene
3′,5′ diacetoxy 2,4 diisopropoxy stilbene
3′,5′ diacetoxy 5 formyl 2,4 diisopropoxy stilbene
3′,5′ dihydroxy 2,4 diisopropoxy stilbene
5 carboxy 2,3′,4,5′ tetrahydroxy stilbene
5 formyl 2,3′,4,5′ tetrahydroxy stilbene
Ascorbic acid
Dimethyl sulfoxide
Oxyresveratrol
Resveratrol
Trolox C
Unclassified drug
Animal cell
Article
Cell viability
Chemical structure
Concentration (parameters)
Controlled study
Drug effect
Nerve cell
Nitroblue tetrazolium test
Nonhuman
Phase contrast microscopy
Synthesis
XTT assay
2′ formyl 2,3′,4,5′ tetrahydroxystilbene
2′ formyl 2,3′,4,5′ tetraisopropoxy stilbene
3′,5′ diacetoxy 2,4 diisopropoxy stilbene
3′,5′ diacetoxy 5 formyl 2,4 diisopropoxy stilbene
3′,5′ dihydroxy 2,4 diisopropoxy stilbene
5 carboxy 2,3′,4,5′ tetrahydroxy stilbene
5 formyl 2,3′,4,5′ tetrahydroxy stilbene
Ascorbic acid
Dimethyl sulfoxide
Oxyresveratrol
Resveratrol
Trolox C
Unclassified drug
Animal cell
Article
Cell viability
Chemical structure
Concentration (parameters)
Controlled study
Drug effect
Nerve cell
Nitroblue tetrazolium test
Nonhuman
Phase contrast microscopy
Synthesis
XTT assay