Publication:
Biocompatibility study of modified injectable hyaluronic acid hydrogel with mannitol/BSA to alveolar bone cells

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

8853282

DOI

10.1177/0885328220971746

Other identifier(s)

2-s2.0-85095454840

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Biomaterials Applications. Vol 35, No.10 (2021), p.1294-1303

Suggested Citation

Areevijit K., Dhanesuan N., Luckanagul J.A., Rungsiyanont S. Biocompatibility study of modified injectable hyaluronic acid hydrogel with mannitol/BSA to alveolar bone cells. Journal of Biomaterials Applications. Vol 35, No.10 (2021), p.1294-1303. doi:10.1177/0885328220971746 Retrieved from: https://hdl.handle.net/20.500.14740/7825

Title

Biocompatibility study of modified injectable hyaluronic acid hydrogel with mannitol/BSA to alveolar bone cells

Author(s)

Areevijit K.
 Dhanesuan N.
 Luckanagul J.A.
 Rungsiyanont S.

Abstract

The quality and quantity of bone are crucial to the success of dental implant treatment. Recently, bone grafting materials have reached some limitations. This study aimed to evaluate the biocompatibility of novel drug delivery material, injectable methacrylated hyaluronic acid hydrogel incorporated with different ratios of mannitol and BSA (Man/BSA MeHA), to human alveolar bone cells. The three-dimensionally encapsulated cell culture was evaluated with the resazurin cell viability test, alkaline phosphatase activity assay, immunohistochemistry test for collagen type-I synthesis, and cell morphology. The results showed that the encapsulated cells were viable in all four ratios of Man/BSA MeHA hydrogel and the average metabolic rate was not less than the control group. The morphology test showed round shape cells at the upper portion of the hydrogel and fibroblast-like or polygonal shape at the lower portion of hydrogel next to the culture plate. All four groups could express enzyme alkaline phosphatase and collagen type-I. In conclusion, four ratios of Man/BSA MeHA hydrogel were biocompatible with primary human alveolar bone cells. © The Author(s) 2020.

Subject(s)

Biocompatibility
 Bone
 Cell culture
 Collagen
 Controlled drug delivery
 Hyaluronic acid
 Hydrogels
 Morphology
 Organic acids
 Phosphatases
 Polyols
 ALkaline phosphatase
 Alkaline phosphatase activity
 Bone grafting materials
 Drug delivery materials
 Encapsulated cell
 Hyaluronic acid hydrogels
 Immunohistochemistry
 Methacrylated hyaluronic acids
 Cells
 Alkaline phosphatase
 Collagen type 1
 Hyaluronic acid derivative
 Hydrogel
 Mannitol
 Biomaterial
 Bovine serum albumin
 Hyaluronic acid
 Mannitol
 Alveolar bone
 Article
 Biocompatibility
 Bone cell
 Cell culture
 Cell encapsulation
 Cell metabolism
 Cell shape
 Cell structure
 Cell viability
 Controlled study
 Enzyme assay
 Human
 Human cell
 Immunohistochemistry
 Priority journal
 Protein expression
 Protein synthesis
 Adult
 Animal
 Bone
 Bone development
 Bovine
 Cell differentiation
 Cell survival
 Chemistry
 Cytology
 Drug effect
 Hydrogel
 Metabolism
 Middle aged
 Pharmacology
 Adult
 Animals
 Biocompatible Materials
 Bone and Bones
 Cattle
 Cell Differentiation
 Cell Survival
 Cells, Cultured
 Humans
 Hyaluronic Acid
 Hydrogels
 Mannitol
 Middle Aged
 Osteogenesis
 Serum Albumin, Bovine

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URI

https://hdl.handle.net/20.500.14740/7825

Collections

Scopus: Year 1983-2021

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