Publication:
Comparison between R-phycocyanin-labeled and R-phycoerythrin-labeled monoclonal antibody (MAb) probes for the detection of Entamoeba histolytica trophozoites

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Issued Date

2001

Resource Type

Review

File Type

application/pdf

ISSN

1251562

Other identifier(s)

2-s2.0-0042832554

Rights Holder(s)

Scopus

Bibliographic Citation

Southeast Asian Journal of Tropical Medicine and Public Health. Vol 32, No.SUPPL. 2 (2001), p.165-171

Suggested Citation

Intrarapuk A., Awakairt S., Thammapalerd N., Mahannop P., Pattanawong U., Suppasiri T. Comparison between R-phycocyanin-labeled and R-phycoerythrin-labeled monoclonal antibody (MAb) probes for the detection of Entamoeba histolytica trophozoites. Southeast Asian Journal of Tropical Medicine and Public Health. Vol 32, No.SUPPL. 2 (2001), p.165-171. Retrieved from: https://hdl.handle.net/20.500.14740/6945

Title

Comparison between R-phycocyanin-labeled and R-phycoerythrin-labeled monoclonal antibody (MAb) probes for the detection of Entamoeba histolytica trophozoites

Author(s)

Intrarapuk A.
 Awakairt S.
 Thammapalerd N.
 Mahannop P.
 Pattanawong U.
 Suppasiri T.

Abstract

A comparison between R-phycocyanin (R-PC)-labeled monoclonal antibody (MAb) probe and R-phycoerythrin (R-PE)-labeled MAb probe for the detection of the three standard reference strains of the cultured-derived Entamoeba histolytica trophozoites, namely HK-9, HM-1:IMSS, and HTH-56-MUTM were evaluated by using direct immunofluorescence antibody (DIFA) assay five times for each strain. Under the blue irradiation of the fluorescent microscope, both R-PC-labeled and R-PE-labeled MAb probes showed consistently greenishyellow trophozoites and golden-orange trophozoites, respectively. The R-PE-labeled MAb probe stained the trophozoites more brightly and clearly than those stained by the R-PC-labeled MAb probe of the same Eh208C2-2MAb. When observed under the green irradiation, both probes showed the same intensity of brightly red color at the trophozoites of all three strains of E. histolytica. The sensitivity of both tests was 100%. Since this Eh208C2-2MAb could recognize specifically E. histolytica pyruvate: ferredoxin oxidoreduclase (PFOR) enzyme, therefore, our two antibody probes would be valuable for use as a rapid, easy and sensitive test for diagnosis of invasive amebiasis. Further applications of these two probes directly onto the fecal sample spots and to more culture-derived strains of E. histolytica/E. dispar of known zymodemes in collaboration with the International Centre for Diarrhoeal Disease Research. Bangladesh (ICDDRB), Dhaka, Bangladesh, are under investigation.

Subject(s)

Diagnostic agent
 Fluorescent dye
 Monoclonal antibody
 Phycocyanin
 Phycoerythrin
 Protozoon antibody
 Amebiasis
 Animal
 Article
 Comparative study
 Dysentery
 Entamoeba histolytica
 Fluorescent antibody technique
 Human
 Immunology
 Isolation and purification
 Methodology
 Mouse
 Parasitology
 Reproducibility
 Sensitivity and specificity
 Animals
 Antibodies, Monoclonal
 Antibodies, Protozoan
 Dysentery, Amebic
 Entamoeba histolytica
 Entamoebiasis
 Fluorescent Antibody Technique, Direct
 Fluorescent Dyes
 Humans
 Mice
 Phycocyanin
 Phycoerythrin
 Reproducibility of Results
 Sensitivity and Specificity

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URI

https://hdl.handle.net/20.500.14740/6945

Collections

Scopus: Year 1983-2021

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Review

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