Publication:
An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways

0
0

Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

9254692

DOI

10.1007/s10787-019-00677-3

Other identifier(s)

2-s2.0-85076098030

Rights Holder(s)

Scopus

Bibliographic Citation

Inflammopharmacology. Vol 28, No.4 (2020), p.1027-1037

Suggested Citation

Pranweerapaiboon K., Apisawetakan S., Nobsathian S., Itharat A., Sobhon P., Chaithirayanon K. An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways. Inflammopharmacology. Vol 28, No.4 (2020), p.1027-1037. doi:10.1007/s10787-019-00677-3 Retrieved from: https://hdl.handle.net/20.500.14740/4461

Title

An ethyl-acetate fraction of Holothuria scabra modulates inflammation in vitro through inhibiting the production of nitric oxide and pro-inflammatory cytokines via NF-κB and JNK pathways

Author(s)

Pranweerapaiboon K.
 Apisawetakan S.
 Nobsathian S.
 Itharat A.
 Sobhon P.
 Chaithirayanon K.

Abstract

Sea cucumber, Holothuria scabra, is an echinoderm marine animal that has long been used as a traditional therapeutic in various diseases due to its chemical composition and protein enrichment. Many researchers have extensively studied the efficacy of sea cucumber extracts for many health benefits in recent years. Inflammation is a complex process involved in pro-/anti-inflammatory cytokine products. However, the role of the H. scabra extracts in anti-inflammation and its molecular regulations has not been apparently elucidated yet. In this study, we investigated the anti-inflammatory effect of H. scabra extracts by using lipopolysaccharide (LPS) from E. coli to induce an inflammatory response in RAW264.7 macrophage. It was found that ethyl acetate fraction of H. scabra extracts (EAHS) inhibited pro-inflammatory cytokines synthesis at both the transcriptional and translational levels, notably nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2). In addition, EAHS was able to downregulate IκB/NF-κB, and JNK expressions. These effects may be influenced by high contents of phenolic compound and triterpene glycosides in EAHS. Therefore, EAHS might have the potential to be developed as a natural anti-inflammatory agent. © 2019, Springer Nature Switzerland AG.

Subject(s)

Acetic acid ethyl ester
 Animal extract
 Antiinflammatory agent
 Butanol
 Cytokine
 Gallic acid
 Glycoside
 Hexane
 Holothuria scabra extract
 I kappa B
 Immunoglobulin enhancer binding protein
 Inducible nitric oxide synthase
 Interleukin 1beta
 Janus kinase
 Lipopolysaccharide
 Messenger RNA
 Mitogen activated protein kinase p38
 Nitric oxide
 Phenol derivative
 Prostaglandin E2
 STAT3 protein
 Terpene
 Triterpene
 Tumor necrosis factor
 Unclassified drug
 Animal cell
 Antiinflammatory activity
 Article
 Cell viability assay
 Comparative study
 Controlled study
 Cytokine production
 Cytokine release
 Cytotoxicity
 Down regulation
 Drug megadose
 Enzyme linked immunosorbent assay
 Holothuria
 Holothuria scabra
 In vitro study
 MRNA expression level
 MTT assay
 Nonhuman
 Priority journal
 Protein expression
 Protein phosphorylation
 Proton nuclear magnetic resonance
 RAW 264.7 cell line

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4461

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By