Publication: A whole blood lymphocyte proliferation assay in healthy thais: Comparison of heparinized blood and acid citrate dextrose blood
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Issued Date
2010
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-79952262665
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 93, No.SUPPL 2 (2010), p.S21-S26
Suggested Citation
Chuenchitra T., Sukwit S., Chaitaveep P., Kuvanont S., Amlee P., Songprasom K., Tabprasit S., De Souza M., Srisurapanon S., Sirisopana N., Nitayaphan S. A whole blood lymphocyte proliferation assay in healthy thais: Comparison of heparinized blood and acid citrate dextrose blood. Journal of the Medical Association of Thailand. Vol 93, No.SUPPL 2 (2010), p.S21-S26. Retrieved from: https://hdl.handle.net/20.500.14740/7487
Abstract
The lymphocyte proliferation assay (LPA) is a technique to determine T-lymphocyte functions in vitro. The standard LPA using peripheral blood mononuclear cells (PBMC) separated from heparinized blood requires a large blood sample, time consuming and expensive. It is more useful if acid citrate dextrose (ACD) blood could be used not only for LPA but also for other purposes. To determine whether whole blood composing between heparinized blood and ACD blood could be substituted for standard LPA using PBMC. Heparinized and ACD blood of 35 healthy Thai blood donors were studied herein. PBMC separated by density gradient centrifugation and diluted heparinized and ACD blood were used to test and compare for lymphoproliferative responses to phytohemagglutinin (PHA), pokeweed mitogen (PWM), and tetanus toxoid. A stimulation index (SI) for each mitogen or antigen was calculated. All Thai blood donors demonstrated positive proliferative responses to PHA and PWM by using PBMC and whole blood culture assays from both heparinized and ACD blood. However, the difference in the frequency of positive proliferative responses to tetanus toxoid by using PBMC and whole blood culture assays was significant. Nevertheless, no significant difference in frequency of positive responses to tetanus toxoid between heparinized and ACD blood was observed. This results suggested that no significant difference between using heparinized and ACD blood in standard LPA using PBMC. However, the whole blood LPA for measuring mitogen induced lymphoproliferation could be substituted for standard LPA from heparinized and ACD blood. Whole blood LPA is easy, rapid, and more cost effective than PBMC culture assay. Thus, it would be applicable in a clinical laboratory as well as in research setting.
Subject(s)
Acid citrate dextrose
Anticoagulant agent
Citric acid
Drug derivative
Glucose
Heparin
Phytohemagglutinin
Pokeweed mitogen
Tetanus toxoid
Adult
Article
Asian
Blood
Comparative study
Density gradient centrifugation
Drug effect
Female
Human
Immunological procedures
Immunology
Lymphocyte activation
Male
T lymphocyte
Adult
Anticoagulants
Asian Continental Ancestry Group
Centrifugation, Density Gradient
Citric Acid
Female
Glucose
Heparin
Humans
Immunologic Tests
Lymphocyte Activation
Male
Phytohemagglutinins
Pokeweed Mitogens
T-Lymphocytes
Tetanus Toxoid
Anticoagulant agent
Citric acid
Drug derivative
Glucose
Heparin
Phytohemagglutinin
Pokeweed mitogen
Tetanus toxoid
Adult
Article
Asian
Blood
Comparative study
Density gradient centrifugation
Drug effect
Female
Human
Immunological procedures
Immunology
Lymphocyte activation
Male
T lymphocyte
Adult
Anticoagulants
Asian Continental Ancestry Group
Centrifugation, Density Gradient
Citric Acid
Female
Glucose
Heparin
Humans
Immunologic Tests
Lymphocyte Activation
Male
Phytohemagglutinins
Pokeweed Mitogens
T-Lymphocytes
Tetanus Toxoid