Publication:
Four novel PYFs: Members of NPY/PP peptide superfamily from the eyestalk of the giant tiger prawn Penaeus monodon

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

1969781

DOI

10.1016/S0196-9781(02)00176-6

Other identifier(s)

2-s2.0-0036849337

Rights Holder(s)

Scopus

Bibliographic Citation

Peptides. Vol 23, No.11 (2002), p.1895-1906

Suggested Citation

Sithigorngul P., Pupuem J., Krungkasem C., Longyant S., Panchan N., Chaivisuthangkura P., Sithigorngul W., Petsom A. Four novel PYFs: Members of NPY/PP peptide superfamily from the eyestalk of the giant tiger prawn Penaeus monodon. Peptides. Vol 23, No.11 (2002), p.1895-1906. doi:10.1016/S0196-9781(02)00176-6 Retrieved from: https://hdl.handle.net/20.500.14740/6691

Title

Four novel PYFs: Members of NPY/PP peptide superfamily from the eyestalk of the giant tiger prawn Penaeus monodon

Author(s)

Sithigorngul P.
 Pupuem J.
 Krungkasem C.
 Longyant S.
 Panchan N.
 Chaivisuthangkura P.
 Sithigorngul W.
 Petsom A.

Abstract

An immunocytochemical method was used for localization of pancreatic polypeptide (PP) immunoreactive substances in the eyestalk of Penaeus monodon using anti-C-terminal hexapeptide of PP (anti-PP6) antiserum. Approximately 200 neuronal cell bodies were recognized in the ganglia between the medulla interna (MI) and medulla terminalis (MT) and surrounding MT in conjunction with the neuronal processes in medulla externa (ME), MI, MT and sinus gland. About half of the PP immunoreactive neurons were also recognized by a combination of three monoclonal antibodies raised against FMRFamide-like peptides. Isolation of the PP immunoreactive substances from the eyestalk was performed using 7500 eyestalks extracted in methanol/acetic acid/water (90/1/9) followed by five to six steps of RP-HPLC separation. Dot-ELISA with anti-PP6 antiserum was used to monitor PP-like substances in various fractions during the purification processes. Four new sequences of one hexapeptide; RARPRFamide, and three nonapeptides; YSQVSRPRFamide, YAIAGRPRFamide and YSLRARPRFamide were identified, and named as Pem-PYF1-4 due to their structural similarity to the PYF found in squid Loligo vulgaris. Each of the new peptides shares four to seven common residues with the C-terminus of the squid PYF and with the NPFs found in other invertebrates. The NPY/PP superfamily as well as the FMRFamide peptide family may be present throughout vertebrates and invertebrates. © 2002 Elsevier Science Inc. All rights reserved.

Subject(s)

Acetic acid
 Amide
 Arginylalanylarginylprolylarginylphenylalaninamide
 Hexapeptide
 Methanol
 Monoclonal antibody
 Neuropeptide
 Neuropeptide Y
 Nonapeptide
 Pancreas polypeptide
 Phenylalanylmethionylarginylphenylalaninamide like peptide
 Tyrosylalanylisoleucylalanylglycylarginylprolylarginylphenylalaninamide
 Tyrosylphenylalanine
 Tyrosylserylglutaminylvalylserylarginylprolylarginylphenylalaninamide
 Tyrosylserylleucylarginylalanylarginylprolylarginylphenylalaninamide
 Unclassified drug
 Water
 Neuropeptide Y
 Amino acid sequence
 Animal tissue
 Article
 Brain region
 Carboxy terminal sequence
 Controlled study
 Enzyme linked immunosorbent assay
 Ganglion
 High performance liquid chromatography
 Immunocompetent cell
 Immunocytochemistry
 Invertebrate
 Nonhuman
 Perikaryon
 Priority journal
 Protein analysis
 Protein family
 Protein isolation
 Protein localization
 Protein purification
 Protein structure
 Sequence analysis
 Animal
 Chemistry
 Eye
 Immunohistochemistry
 Isolation and purification
 Molecular genetics
 Penaeidae
 Sequence homology
 Cephalopoda
 Invertebrata
 Loligo
 Loligo vulgaris
 Monodon
 Panthera tigris
 Penaeus monodon
 Vertebrata
 Amino Acid Sequence
 Animals
 Enzyme-Linked Immunosorbent Assay
 Eye
 Immunohistochemistry
 Molecular Sequence Data
 Neuropeptide Y
 Penaeidae
 Sequence Homology, Amino Acid

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URI

https://hdl.handle.net/20.500.14740/6691

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Scopus: Year 1983-2021

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