Publication:
Antiproliferative effect of α-mangostin on canine osteosarcoma cells

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

345288

DOI

10.1016/j.rvsc.2012.01.015

Other identifier(s)

2-s2.0-84863108518

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Research in Veterinary Science. Vol 93, No.2 (2012), p.788-794

Suggested Citation

Krajarng A., Nilwarankoon S., Suksamrarn S., Watanapokasin R. Antiproliferative effect of α-mangostin on canine osteosarcoma cells. Research in Veterinary Science. Vol 93, No.2 (2012), p.788-794. doi:10.1016/j.rvsc.2012.01.015 Retrieved from: https://hdl.handle.net/20.500.14740/6951

Title

Antiproliferative effect of α-mangostin on canine osteosarcoma cells

Author(s)

Krajarng A.
 Nilwarankoon S.
 Suksamrarn S.
 Watanapokasin R.

Abstract

Osteosarcoma is the most frequently diagnosed primary bone tumor in dog. Since chemotherapeutics are quite limited due to high cost and severe toxicity, therefore, the ultimate goal is to discover cost-effective therapeutics with less toxicity. We have studied the effect of α-mangostin, a xanthone derivative isolated from pericarp of mangosteen (Garcinia mangostana Linn.) in canine osteosarcoma, D-17 cells. The results showed that α-mangostin induced antiproliferation with IC50 at 15μg/ml. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that α-mangostin could induce nuclear condensation and fragmentation, typically seen in apoptosis. Cell cycle analysis demonstrated that α-mangostin induced sub-G1 peak. In addition, α-mangostin also induced membrane flipping of the phosphatidylserine and the loss of mitochondrial membrane potential in D-17 cells. In conclusion, α-mangostin, induced apoptotic cell death against canine osteosarcoma D-17 cells, could be a potential candidate for preventive and therapeutic application for bone cancer treatment in dogs. © 2012 Elsevier Ltd.

Subject(s)

Alpha mangostin
 Antineoplastic agent
 DNA
 Hoe 33342
 Phosphatidylserine
 Unclassified drug
 Xanthone derivative
 Animal cell
 Antiproliferative activity
 Apoptosis
 Article
 Cell cycle G1 phase
 Cell membrane
 Cell nucleus fragmentation
 Cell strain D 17
 Cellular parameters
 Concentration response
 Controlled study
 DNA fragmentation
 Dog
 Drug cytotoxicity
 Drug isolation
 Flow cytometry
 Garcinia mangostana
 Gel electrophoresis
 IC 50
 Mitochondrial membrane potential
 Nonhuman
 Nuclear condensation
 Nucleosome
 Osteosarcoma
 Osteosarcoma cell
 Pericarp
 Phospholipid metabolism
 Staining
 Animals
 Antineoplastic Agents, Phytogenic
 Cell Cycle
 Cell Line, Tumor
 Cell Proliferation
 Cell Survival
 Dogs
 Garcinia mangostana
 Osteosarcoma
 Xanthones
 Canis familiaris
 Garcinia mangostana

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URI

https://hdl.handle.net/20.500.14740/6951

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Scopus: Year 1983-2021

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