Publication:
Simple immunoblot and immunohistochemical detection of Penaeus stylirostris densovirus using monoclonal antibodies to viral capsid protein expressed heterologously

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Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2009.07.026

Other identifier(s)

2-s2.0-70349755387

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Virological Methods. Vol 162, (2009), p.126-132

Suggested Citation

Sithigorngul P., Hajimasalaeh W., Longyant S., Sridulyakul P., Rukpratanporn S., Chaivisuthangkura P. Simple immunoblot and immunohistochemical detection of Penaeus stylirostris densovirus using monoclonal antibodies to viral capsid protein expressed heterologously. Journal of Virological Methods. Vol 162, (2009), p.126-132. doi:10.1016/j.jviromet.2009.07.026 Retrieved from: https://hdl.handle.net/20.500.14740/3702

Title

Simple immunoblot and immunohistochemical detection of Penaeus stylirostris densovirus using monoclonal antibodies to viral capsid protein expressed heterologously

Author(s)

Sithigorngul P.
 Hajimasalaeh W.
 Longyant S.
 Sridulyakul P.
 Rukpratanporn S.
 Chaivisuthangkura P.

Abstract

Penaeus stylirostris densovirus (PstDNV), called formerly infectious hypodermal and hematopoietic necrosis virus (IHHNV), is an important shrimp pathogen which can cause mortality in the blue shrimp Penaeus (Litopenaeus) stylirostris and stunting in the whiteleg shrimp Penaeus (Litopenaeus) vannamei. Five monoclonal antibodies (MAbs) were produced against the 37 kDa capsid protein 3 (CP3) of PstDNV expressed heterologously in the form of a fusion protein with glutathione-S-transferase called GST-CP3. All MAbs belonged to the IgG2b subclass and could bind to GST-CP3 at 300 pg/spot in immunodot-blot tests. They could detect CP3 in naturally infected shrimp extracts by Western blotting and dot blotting and in shrimp tissues by immunohistochemistry without cross-reactivity to extracts from uninfected shrimps or shrimps infected with several other viruses. Although dot blot assay sensitivity was approximately 1000 times lower than that of one step PCR for PstDNV, it easily detected PstDNV infections in field samples of Penaeus monodon and Penaeus vannamei. © 2009 Elsevier B.V. All rights reserved.

Subject(s)

Capsid protein
 Glutathione transferase
 Hybrid protein
 Immunoglobulin G2b
 Monoclonal antibody
 Article
 Controlled study
 Cross reaction
 Heterologous expression
 Immunoblotting
 Immunohistochemistry
 Infectious hematopoietic necrosis virus
 Mortality
 Nonhuman
 Penaeus stylirostris densovirus
 Polymerase chain reaction
 Priority journal
 Protein expression
 Rhabdovirus
 Sensitivity and specificity
 Shrimp
 Virus detection
 Western blotting
 Animals
 Antibodies, Monoclonal
 Antibodies, Viral
 Capsid Proteins
 Densovirus
 Glutathione Transferase
 Immunoblotting
 Immunohistochemistry
 Penaeidae
 Recombinant Fusion Proteins
 Sensitivity and Specificity
 Decapoda (Crustacea)
 Densovirus
 Infectious hypodermal and hematopoietic necrosis virus
 Litopenaeus stylirostris
 Litopenaeus vannamei
 Miridae
 Penaeus monodon

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URI

https://hdl.handle.net/20.500.14740/3702

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Scopus: Year 1983-2021

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