Publication: Characterization and Whole-Genome Sequencing of Phytobacter palmae WL65, a Plant Growth-Promoting Rhizobacterium First Isolated from Rice Rhizosphere Soil in Thailand
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Issued Date
2025-04-01
Resource Type
eISSN
20770472
Scopus ID
2-s2.0-105002297578
Journal Title
Agriculture (Switzerland)
Volume
15
Issue
7
Rights Holder(s)
SCOPUS
Bibliographic Citation
Agriculture (Switzerland) Vol.15 No.7 (2025)
Suggested Citation
Thamvithayakorn P., Phosri C., Vishal V., Suwannasai N. Characterization and Whole-Genome Sequencing of Phytobacter palmae WL65, a Plant Growth-Promoting Rhizobacterium First Isolated from Rice Rhizosphere Soil in Thailand. Agriculture (Switzerland) Vol.15 No.7 (2025). doi:10.3390/agriculture15070707 Retrieved from: https://hdl.handle.net/20.500.14740/20524
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Abstract
Phytobacter palmae WL65, isolated from the rice rhizosphere, was confirmed as P. palmae through whole-genome analysis. WL65 exhibited key plant growth-promoting (PGP) characteristics, including nitrogen fixation (nifA, nifB, nifD, nifE, nifF, nifH, nifJ, nifK, nifL, nifS, nifU, nifW, and nifX), phosphate solubilization (pstA, pstB, pstC, pstS, phnC, phnD, phnE, and phnV), siderophore production (fhuA, fhuB, fhuC, fhuD, fhuF, feoA, feoB, feoC, acrA, acrB, acrE, acrR, and acrZ), and phytohormone biosynthesis (trpA, trpB, trpC, trpE, trpGD, trpR, and trpS). WL65 also contains an enterobactin biosynthetic gene cluster, essential for iron acquisition and enhancing both bacterial survival and plant growth. This study provides the first genomic insights into the PGP characteristics of P. palmae. The application of WL65 in rice cultivation as a biostimulant resulted in effective root colonization, supported by biofilm formation genes (pgaA, pgaB, pgaC), which enhance bacterial adhesion. The treatment significantly improved rice growth, increasing plant height (5.8%), panicle length (10.2%), and seed yield (34.5%). Soil analysis revealed improved nutrient availability, including increased organic matter (21%), phosphorus (38.4%), potassium (29.8%), and calcium (27%) levels. These findings suggest that WL65 is a promising biofertilizer candidate for improving soil fertility and nutrient uptake in sustainable agriculture.