Publication:
Two novel antimicrobial defensins from rice identified by gene coexpression network analyses

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Issued Date

2016

Resource Type

Article

File Type

application/pdf

ISSN

1969781

DOI

10.1016/j.peptides.2016.07.005

Other identifier(s)

2-s2.0-84982291672

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Peptides. Vol 84, (2016), p.7-16

Suggested Citation

Tantong S., Pringsulaka O., Weerawanich K., Meeprasert A., Rungrotmongkol T., Sarnthima R., Roytrakul S., Sirikantaramas S. Two novel antimicrobial defensins from rice identified by gene coexpression network analyses. Peptides. Vol 84, (2016), p.7-16. doi:10.1016/j.peptides.2016.07.005 Retrieved from: https://hdl.handle.net/20.500.14740/5145

Title

Two novel antimicrobial defensins from rice identified by gene coexpression network analyses

Author(s)

Tantong S.
 Pringsulaka O.
 Weerawanich K.
 Meeprasert A.
 Rungrotmongkol T.
 Sarnthima R.
 Roytrakul S.
 Sirikantaramas S.

Abstract

Defensins form an antimicrobial peptides (AMP) family, and have been widely studied in various plants because of their considerable inhibitory functions. However, their roles in rice (Oryza sativa L.) have not been characterized, even though rice is one of the most important staple crops that is susceptible to damaging infections. Additionally, a previous study identified 598 rice genes encoding cysteine-rich peptides, suggesting there are several uncharacterized AMPs in rice. We performed in silico gene expression and coexpression network analyses of all genes encoding defensin and defensin-like peptides, and determined that OsDEF7 and OsDEF8 are coexpressed with pathogen-responsive genes. Recombinant OsDEF7 and OsDEF8 could form homodimers. They inhibited the growth of the bacteria Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, and Erwinia carotovora subsp. atroseptica with minimum inhibitory concentration (MIC) ranging from 0.6 to 63 μg/mL. However, these OsDEFs are weakly active against the phytopathogenic fungi Helminthosporium oryzae and Fusarium oxysporum f.sp. cubense. This study describes a useful method for identifying potential plant AMPs with biological activities. © 2016 Elsevier Inc.

Subject(s)

Defensin
 Homodimer
 Polypeptide antibiotic agent
 Antiinfective agent
 Cysteine
 Defensin
 Peptide
 Antibacterial activity
 Antifungal activity
 Article
 Bipolaris oryzae
 Controlled study
 Fusarium oxysporum f. sp. cubense
 Gene
 Gene coexpression network analysis
 Gene expression
 Genetic analysis
 Minimum inhibitory concentration
 Nonhuman
 OsDEF7 gene
 OsDEF8 gene
 Pectobacterium atrosepticum
 Priority journal
 Recombinant gene
 Rice
 Xanthomonas oryzae pv. oryzae
 Xanthomonas oryzae pv. oryzicola
 Computer simulation
 Fusarium
 Gene expression regulation
 Genetics
 Helminthosporium
 Isolation and purification
 Microbiology
 Oryza
 Pathogenicity
 Plant disease
 Prevention and control
 Xanthomonas
 Anti-Infective Agents
 Computer Simulation
 Cysteine
 Defensins
 Fusarium
 Gene Expression Regulation, Plant
 Helminthosporium
 Oryza
 Peptides
 Plant Diseases
 Xanthomonas

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URI

https://hdl.handle.net/20.500.14740/5145

Collections

Scopus: Year 1983-2021

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