Publication:
Effects of phikud navakot extract on myocardial ischemia/reperfusion injury in rats

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Issued Date

2015

Resource Type

Article

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application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84957616565

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 98, (2015), p.S39-S47

Suggested Citation

Kengkoom K., Sirimontaporn A., Sotanaphun U., Gerdprasert O., Nusuetrong P. Effects of phikud navakot extract on myocardial ischemia/reperfusion injury in rats. Journal of the Medical Association of Thailand. Vol 98, (2015), p.S39-S47. Retrieved from: https://hdl.handle.net/20.500.14740/6064

Title

Effects of phikud navakot extract on myocardial ischemia/reperfusion injury in rats

Author(s)

Kengkoom K.
 Sirimontaporn A.
 Sotanaphun U.
 Gerdprasert O.
 Nusuetrong P.

Abstract

Background: Phikud Navakot (PN) is a set of nine medicinal plants and the main ingredient of “Yahom Navakot”, a traditional Thai herbal formula for treatment of cardiovascular symptoms. Objective: To investigate the cardioprotective effects of PN on myocardial ischemia/reperfusion (IR) in male Sprague Dawley rats. Material and Method: Rats were randomly divided into 7 groups: sham, IR, and IR orally pretreated with PN (10, 50, 100, 200, and 400 mg/kg BW) for 7 days. After treatment, IR induction was performed by left coronary artery (LCA) ligation for 30 min, followed by reperfusion for 24 h. At the end of the experiment, blood was collected for hematological and biochemical parameters, and hearts were immediately removed for histopathological examination and Western blot analysis. Results: IR induction caused ST elevation in the electrocardiogram and an increase in serum troponin I (TnI), confirming myocardial damage. In addition, histopathological changes of ischemic myocardium showed inflammation, infiltration, and edema. Oral administration of PN (10, 50, 100, 200, and 400 mg/kg BW) for 7 days prior to IR simulation showed no change on serum TnI and histopathology of cardiac tissues, when compared to IR group. However, Western blot analysis showed that IR rats pretreated with PN (10 mg/kg BW) significantly increased (p<0.05) pERK/ERK ratio, meanwhile pretreated with PN (50-200 mg/kg BW) up-regulated (p<0.05) the protein expression of HO-1, when compared with IR group. Conclusion: The present study implied that 7-day pretreatment of PN failed to protect cardiac tissues against IR injury induced by LCA ligation. Investigation at molecular level found however that PN up-regulated the expression of protective proteins pERK/ERK ratio and HO-1 in cardiac tissues, suggesting molecular mechanism of PN in cardioprotection against IR injury. © 2015, Medical Association of Thailand. All rights reserved.

Subject(s)

Heme oxygenase 1
 Lactate dehydrogenase
 Mitogen activated protein kinase
 Nitrous oxide
 Phikud navakot extract
 Plant extract
 Protein kinase B
 Troponin I
 Unclassified drug
 Phikud navakot
 Plant extract
 Adult
 Animal experiment
 Animal model
 Animal tissue
 Article
 Blood sampling
 Controlled study
 Coronary artery ligation
 Electrocardiography
 Heart muscle injury
 Heart muscle ischemia
 Heart protection
 Histopathology
 Immunoassay
 Left coronary artery
 Male
 Nonhuman
 Protein expression
 Rat
 ST segment elevation
 Upregulation
 Western blotting
 Animal
 Chemistry
 Heart muscle
 Medicinal plant
 Myocardial Reperfusion Injury
 Pathology
 Sprague Dawley rat
 Animals
 Electrocardiography
 Male
 Myocardial Reperfusion Injury
 Myocardium
 Plant Extracts
 Plants, Medicinal
 Rats
 Rats, Sprague-Dawley

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URI

https://hdl.handle.net/20.500.14740/6064

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Scopus: Year 1983-2021

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