Publication:
Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

13860291

Other identifier(s)

2-s2.0-42049099042

Rights Holder(s)

Scopus

Bibliographic Citation

Clinical Hemorheology and Microcirculation. Vol 38, No.4 (2008), p.235-243

Suggested Citation

Kasiyaphat A., Siriviriyakul P., Patumraj S. Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats. Clinical Hemorheology and Microcirculation. Vol 38, No.4 (2008), p.235-243. Retrieved from: https://hdl.handle.net/20.500.14740/4048

Title

Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats

Author(s)

Kasiyaphat A.
 Siriviriyakul P.
 Patumraj S.

Abstract

This study was aimed to examine effects of genistein on leukocyte adhesion in femur microcirculation in relation to bone-loss induced in ovariectomized female rats. Sixty-four female Wistar rats were divided into 4 groups: sham (daily treated with vehicle; DMSO, sc; 100 μl/day), ovariectomized rat treated with vehicle (OVXveh), 17β-estradiol treated-ovariectomized rat (OVXE2, 5 μg/kg/day, sc) and genistein treated-ovariectomized rat (0.25 mg/kg/day, sc; OVXgen). One and three weeks after the ovariectomy, blood flow perfusion (BF) in femur tissue was measured using laser Doppler flowmetry. Leukocyte adhesion in femur venules (15-30 μm in diameter) of each group was evaluated by intravital fluorescence videomicroscopy. The bone mineral content (BMC) was measured and expressed in terms of ratio of ash-to-dry matter weight. Serum osteocalcin and alkaline phosphatase levels were determined using chemiluminescence immunoassay. In both one and three week-OVXveh, leukocyte adhesion increased significantly, compared to their age-matched sham groups, but it decreased significantly in OVXgen, compared to OVXveh (p<0.05). In three week-OVXgen, both BF and BMC increased significantly, but osteocalcin and alkaline phosphatase levels decreased, compared to those of three week-OVXveh. In conclusion, genistein supplementation could effectively prevent bone-loss and microvascular endothelial dysfunction induced by estrogen deficiency. © 2008 - IOS Press and the authors. All rights reserved.

Subject(s)

Alkaline phosphatase
 Dimethyl sulfoxide
 Estradiol
 Genistein
 Osteocalcin
 Alkaline phosphatase blood level
 Animal experiment
 Animal tissue
 Article
 Bone blood flow
 Bone mineral
 Chemoluminescence
 Controlled study
 Drug effect
 Female
 Femur
 Fluorescence
 Immunoassay
 Laser Doppler flowmetry
 Leukocyte adherence
 Microcirculation
 Nonhuman
 Ossification
 Osteolysis
 Ovariectomy
 Rat
 Animals
 Bone and Bones
 Bone Density
 Cell Adhesion
 Disease Models, Animal
 Female
 Femur
 Genistein
 Leukocytes
 Microscopy, Video
 Ovariectomy
 Phytoestrogens
 Rats
 Rats, Wistar
 Venules

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URI

https://hdl.handle.net/20.500.14740/4048

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Scopus: Year 1983-2021

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