Publication: Anti-inflammatory effect of probiotic Lactobacillus paracasei MSMC39-1 on alcohol-induced hepatitis in rats
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
22313354
Other identifier(s)
2-s2.0-85104269935
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Applied Pharmaceutical Science. Vol 11, No.4 (2021), p.46-56
Suggested Citation
Ladda B., Tangteerawatana P., Padungchaichot P., Pradidarcheep W., Kasorn A., Taweechotipatr M. Anti-inflammatory effect of probiotic Lactobacillus paracasei MSMC39-1 on alcohol-induced hepatitis in rats. Journal of Applied Pharmaceutical Science. Vol 11, No.4 (2021), p.46-56. doi:10.7324/JAPS.2021.110407 Retrieved from: https://hdl.handle.net/20.500.14740/7842
Abstract
Probiotics are good microbiota which are able to promote the health of host immunomodulation. In this study, we prepared four antigens of Lactobacillus paracasei MSMC39-1 for the detection of tumor necrosis factor-a (TNFa) secretion in hepatocellular carcinoma cell line (HepG-2). We found that viable cells and heat-killed cells of L. paracasei MSMC39-1 exhibited strong TNF-a secretion inhibitors. Viable cells of L. paracasei MSMC39-1 treatment significantly reduced serum level of aspartate aminotransferase in hepatitis rats. TNF-a secretion in liver tissues of hepatitis rats in probiotic L. paracasei MSMC39-1 treatment was significantly suppressed. Liver histopathology examination of alcohol-induced hepatitis indicated fat accumulation in the hepatocytes. Interestingly, liver damage in hepatitis rats was improved by probiotic L. paracasei MSMC39-1. In addition, L. paracasei MSMC39-1 alleviated colon inflammation in alcohol-induced hepatitis rats. Administration of L. paracasei MSMC39-1 modulated gut microbiota by increasing the number of genera Lactobacillus and Bifidobacterium in alcohol-induced hepatitis rats. We conclude that L. paracasei MSMC39-1 has potent anti-inflammatory effects on HepG-2 cell line and improves hepatitis, colon inflammation, and modulates gut microbiota. Thus L. paracasei MSMC39-1 is able to protect against alcoholic hepatitis. It can also be used as an alternative treatment in other inflammation diseases and applied in nutraceutical and dietary supplement products. © 2021. Boonyarut Ladda et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). All rights reserved.