Publication: Development and assessment of tyrosinase inhibitory activity of liposomes of asparagus racemosus extracts
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
18180876
Other identifier(s)
2-s2.0-84881319110
Rights Holder(s)
Scopus
Bibliographic Citation
Asian Journal of Pharmaceutical Sciences. Vol 8, No.2 (2013), p.134-142
Suggested Citation
Therdphapiyanak N., Jaturanpinyo M., Waranuch N., Kongkaneramit L., Sarisuta N. Development and assessment of tyrosinase inhibitory activity of liposomes of asparagus racemosus extracts. Asian Journal of Pharmaceutical Sciences. Vol 8, No.2 (2013), p.134-142. doi:10.1016/j.ajps.2013.07.017 Retrieved from: https://hdl.handle.net/20.500.14740/6826
Abstract
The purpose of this study was to develop liposomal formulations of Asparagus racemosus root extract (AR1-6) as well as evaluate the physicochemical properties and in vitro tyrosinase inhibitory activity. Liposomes composed of AR1-6 to lipid weight ratio of 1:10 and lecithin (LEC) or Phospholipon® 90G (PC90G) as structural phospholipid at 7:3 molar ratio to CHOL were prepared by various methods, i.e. chloroform-film (CF), reversephase evaporation (REV), polyol dilution (PD), and freeze-drying of monophase solution (MFD) methods. The results revealed that vesicles prepared by CF and MFD were multilamellar whereas those prepared by REV and PD were oligolamellar in nature with particle sizes ranging from 0.26 to 13.83 μm. The zeta potentials were in the range of -1.5 to - 39.3 mV. AR1-6 liposomes with LEC possessed significantly higher entrapment than those with PC90G. The highest entrapment efficiency and in vitro tyrosinase inhibitory activity of 69.08% and 25%, respectively, were obtained from liposomes having LEC and prepared by PD method. The tyrosinase inhibitory activity were in the rank order of LEC > PC90G, and PD > CF > REV > MFD. It could be concluded that the mechanism of vesicle forming in each method of preparation was the key factor influencing physicochemical properties, particularly vesicle type, size, surface charge, and entrapment, which were well correlated with the biological activity.
Subject(s)
Asparagus racemosus extract
Chloroform
Cholesterol
Kojic acid
Levodopa
Liposome
Monophenol monooxygenase
Phosphatidylcholine
Polyol
Article
Asparagus racemosus
Biological activity
Controlled study
Dilution
Drug formulation
Enzyme inhibition
Evaporation
Freeze drying
In vitro study
Particle size
Phospholipid vesicle
Physical chemistry
Plant root
Solvent extraction
Surface charge
Zeta potential
Chloroform
Cholesterol
Kojic acid
Levodopa
Liposome
Monophenol monooxygenase
Phosphatidylcholine
Polyol
Article
Asparagus racemosus
Biological activity
Controlled study
Dilution
Drug formulation
Enzyme inhibition
Evaporation
Freeze drying
In vitro study
Particle size
Phospholipid vesicle
Physical chemistry
Plant root
Solvent extraction
Surface charge
Zeta potential