Publication: An adiponectin-S1P axis protects against lipid induced insulin resistance and cardiomyocyte cell death via reduction of oxidative stress
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0
Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
17437075
Other identifier(s)
2-s2.0-85062325639
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Nutrition and Metabolism. Vol 16, No.1 (2019)
Suggested Citation
Botta A., Liu Y., Wannaiampikul S., Tungtrongchitr R., Dadson K., Park T.-S., Sweeney G. An adiponectin-S1P axis protects against lipid induced insulin resistance and cardiomyocyte cell death via reduction of oxidative stress. Nutrition and Metabolism. Vol 16, No.1 (2019). doi:10.1186/s12986-019-0342-y Retrieved from: https://hdl.handle.net/20.500.14740/5452
Abstract
Background: Adiponectin exerts several beneficial cardiovascular effects, however their specific molecular mechanisms require additional understanding. This study investigated the mechanisms of adiponectin action in the heart during high fat diet (HFD) feeding or in palmitate (PA) treated H9c2 cardiomyoblasts. Methods: 6-week-old male adiponectin knock out (Ad-KO) mice were fed chow or 60% HFD for 6 weeks then received saline or recombinant adiponectin (3μg/g body weight) for an additional 2 weeks. After acute insulin stimulation (4 U/kg), tissue and serum samples were collected for analysis. H9c2 cardiomyocytes were treated ±0.1 mM PA, the adiponectin receptor agonist AdipoRon, or the antioxidant MnTBAP then assays to analyze reactive oxygen species (ROS) production and cell death were conducted. To specifically determine the mechanistic role of S1P, gain and loss of function studies were conducted with adding S1P to cells or the inhibitors THI and SKI-II, respectively. Results: HFD feeding induced cardiac insulin resistance in Ad-KO mice, which was reversed following replenishment of normal circulating adiponectin levels. In addition, myocardial total triglyceride was elevated by HFD and lipidomic analysis showed increased levels of ceramides and sphingosine-1-phosphate (S1P), with only the latter being corrected by adiponectin administration. Similarly, treatment of H9C2 cardiomyoblasts with PA led to a significant increase of intracellular S1P but not in conditioned media whereas AdipoRon significantly increased S1P production and secretion from cells. AdipoRon or the antioxidant MnTBAP significantly reduced PA-induced cell death. Gain and loss of function studies suggested S1P secretion and autocrine receptor activation mediated the effect of AdipoRon to attenuate PA-induced ROS production and cell death. Conclusion: Our data establish adiponectin signaling-mediated increase in S1P secretion as a mechanism via which HFD or PA induced cardiomyocyte lipotoxicity, leading to insulin resistance and cell death, is attenuated. © 2019 The Author(s).
Subject(s)
Adiponectin
Adiponectin receptor 1
Adiponectin receptor 2
Adiporon
Ceramide
Protein inhibitor
Reactive oxygen metabolite
Sphingosine 1 phosphate
Triacylglycerol
Unclassified drug
Animal cell
Animal experiment
Animal model
Article
Autocrine effect
Biosynthesis
Blood analysis
Blood sampling
Cardiac muscle cell
Cell death
Cell protection
Controlled study
Gain of function mutation
Insulin resistance
Intracellular membrane
Knockout mouse
Lipid diet
Loss of function mutation
Male
Mouse
Myoblast
Nonhuman
Oxidative stress
Protein function
Adiponectin receptor 1
Adiponectin receptor 2
Adiporon
Ceramide
Protein inhibitor
Reactive oxygen metabolite
Sphingosine 1 phosphate
Triacylglycerol
Unclassified drug
Animal cell
Animal experiment
Animal model
Article
Autocrine effect
Biosynthesis
Blood analysis
Blood sampling
Cardiac muscle cell
Cell death
Cell protection
Controlled study
Gain of function mutation
Insulin resistance
Intracellular membrane
Knockout mouse
Lipid diet
Loss of function mutation
Male
Mouse
Myoblast
Nonhuman
Oxidative stress
Protein function