Publication:
Antimetastatic potential of N-acetylcysteine on human prostate cancer cells

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876916324

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S56-S62

Suggested Citation

Supabphol A., Supabphol R. Antimetastatic potential of N-acetylcysteine on human prostate cancer cells. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S56-S62. Retrieved from: https://hdl.handle.net/20.500.14740/6868

Title

Antimetastatic potential of N-acetylcysteine on human prostate cancer cells

Author(s)

Supabphol A.
 Supabphol R.

Abstract

Objective: N-acetylcysteine (NAC), is one of the cheapest, safest and widely used over-the-counter-drugs in Thailand. Here the authors examine the antimetastatic potential of NAC on the metastasis of human prostate cancer cells. Material and Method: Cytotoxicity of NAC to human prostate cancer cells, DU145 and PC3, were determined by proliferation assay using the 3-(4, 5-dimethylthiazol, 2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. Cell migration and invasion were assessed by using a chemotaxis chamber containing membrane pre-coated with collagen IV and Matrigel®, respectively. Cell attachment onto the surface of the membrane coated with collagen IV was tested for its adhesion potentiality. Results: NAC could inhibit the growth of DU145 and PC3 cells. Suppression of migration and invasion of both human prostrate cancer cells were observed. Cell attachment to the collagen IV-coated surface was obviously reduced. All inhibitions occurred in a dose-dependent fashion in both cell lines. Conclusion: NAC could have a high potential in attenuating the migration of the human prostate cancer cells from their primary site and their adhesion and invasion to the remote locations. Hence, NAC might suppress the growth of the primary and the secondary tumors. Our findings suggest that NAC had a high possibility to become an antimetastatic agent for testing in clinical trials. Then, NAC might be used clinically as an optional adjuvant therapeutic drug in addition to the conventional standard treatment of human prostate cancer, obtaining a better outcome with the least toxic and affordable substance.

Subject(s)

Acetylcysteine
 Article
 Cancer inhibition
 Cell adhesion
 Cell invasion
 Cell migration
 Cell proliferation
 Cell survival
 Controlled study
 Cytotoxicity
 Enzyme linked immunosorbent assay
 Human
 Human cell
 IC 50
 Metastasis
 Prostate cancer
 Acetylcysteine
 Adenocarcinoma
 Cell Adhesion
 Cell Line, Tumor
 Cell Movement
 Cell Proliferation
 Dose-Response Relationship, Drug
 Free Radical Scavengers
 Humans
 Male
 Neoplasm Invasiveness
 Prostatic Neoplasms

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URI

https://hdl.handle.net/20.500.14740/6868

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Scopus: Year 1983-2021

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