Publication:
Hydroxyxanthone ameliorates IL1β-induced epithelial barrier disruption in colonic-like cells by down-regulation of p-MLC expression

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

17564646

DOI

10.1016/j.jff.2021.104814

Other identifier(s)

2-s2.0-85117701600

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Functional Foods. Vol 87, No. (2021)

Suggested Citation

Chayalak W., Deachapunya C., Suksamran S., Poonyachoti S. Hydroxyxanthone ameliorates IL1β-induced epithelial barrier disruption in colonic-like cells by down-regulation of p-MLC expression. Journal of Functional Foods. Vol 87, No. (2021). doi:10.1016/j.jff.2021.104814 Retrieved from: https://hdl.handle.net/20.500.14740/7819

Title

Hydroxyxanthone ameliorates IL1β-induced epithelial barrier disruption in colonic-like cells by down-regulation of p-MLC expression

Author(s)

Chayalak W.
 Deachapunya C.
 Suksamran S.
 Poonyachoti S.

Abstract

Hydroxyxanthones, the synthetic form of xanthones that predominantly found in mangosteen (Garcinia mangostana), have been indicated as a potent anti-inflammatory action. This study aimed to examine the action of various HDX forms in preventing a tight junction (TJ) barrier disruption induced by TNF-α or IL-1β through the inhibition of p-MLC expression in Caco-2 cell model. TJ barrier function was determined by transepithelial electrical resistance (TER) and flux measurement of fluorescein isothiocyanate-dextran (FD-4). 1-monoHDX or 1,3-diHDX (10 μM) promoted TER but not FD-4 permeability in the absence of cytokines. Pretreatment with 1-monoHDX at 10 μM for 24 h prevented TNF-α or IL-1β effect on TJ permeability. p-MLC protein expression by Western blot analysis, which was induced by IL-1β was inhibited with all HDXs. Tricyclic scaffold as the basic skeleton of all HDXs may be a potential candidate therapeutic for treatment of diseases-related leaky gut. © 2021

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URI

https://hdl.handle.net/20.500.14740/7819

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Scopus: Year 1983-2021

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