Publication:
Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

9396411

DOI

10.1016/j.ejpb.2016.12.017

Other identifier(s)

2-s2.0-85008473077

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

European Journal of Pharmaceutics and Biopharmaceutics. Vol 116, (2017), p.149-154

Suggested Citation

Löwenau L.J., Zoschke C., Brodwolf R., Volz P., Hausmann C., Wattanapitayakul S., Boreham A., Alexiev U., Schäfer-Korting M. Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes. European Journal of Pharmaceutics and Biopharmaceutics. Vol 116, (2017), p.149-154. doi:10.1016/j.ejpb.2016.12.017 Retrieved from: https://hdl.handle.net/20.500.14740/4138

Title

Increased permeability of reconstructed human epidermis from UVB-irradiated keratinocytes

Author(s)

Löwenau L.J.
 Zoschke C.
 Brodwolf R.
 Volz P.
 Hausmann C.
 Wattanapitayakul S.
 Boreham A.
 Alexiev U.
 Schäfer-Korting M.

Abstract

Extrinsic (photo) aging accelerates chronologically aging in the skin due to cumulative UV irradiation. Despite recent insights into the molecular mechanisms of fibroblast aging, age-related changes of the skin barrier function have been understudied. In contrast, the constantly increasing subpopulation of aged patients causes a clinical need for effective and safe (dermatological) treatment. Herein, we reconstructed human epidermis from UVB-irradiated keratinocytes (UVB-RHE). UVB-irradiated keratinocytes show higher activity of senescence associated β-galactosidase, less cell proliferation, and reduced viability. Higher amounts of β-galactosidase are also detectable in UVB-RHE. Moreover, UVB-RHE release more interleukin-1α and -8 into the culture medium and present altered differentiation with a thinner stratum corneum compared to normal RHE. For the first time, the permeation of testosterone and caffeine through UVB-irradiated RHE indicate a clear influence of the UVB stress on the skin barrier function. Impaired barrier function was confirmed by the increased permeation of testosterone and caffeine as well as by the increased penetration of dendritic core-multishell nanocarriers into the constructs. Taken together, UVB-RHE emulate hallmarks of skin aging and might contribute to an improved non-clinical development of medicinal or cosmetic products. © 2016

Subject(s)

Beta galactosidase
 Caffeine
 Interleukin 1alpha
 Interleukin 8
 Nanocarrier
 Testosterone
 Beta galactosidase
 Caffeine
 Drug carrier
 Interleukin 1alpha
 Interleukin 8
 Nanoparticle
 Testosterone
 Aging
 Article
 Cell differentiation
 Cell proliferation
 Cell viability
 Controlled study
 Culture medium
 Human
 Human cell
 Keratinocyte
 Normal human
 Permeability
 Senescence
 Skin permeability
 Stratum corneum
 Ultraviolet B radiation
 Cell culture
 Cell survival
 Cutaneous parameters
 Drug effects
 Epidermis
 Fibroblast
 Keratinocyte
 Metabolism
 Physiology
 Ultraviolet radiation
 Beta-Galactosidase
 Caffeine
 Cell Differentiation
 Cell Proliferation
 Cell Survival
 Cells, Cultured
 Drug Carriers
 Epidermis
 Fibroblasts
 Humans
 Interleukin-1alpha
 Interleukin-8
 Keratinocytes
 Nanoparticles
 Permeability
 Skin Aging
 Testosterone
 Ultraviolet Rays

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URI

https://hdl.handle.net/20.500.14740/4138

Collections

Scopus: Year 1983-2021

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