Publication:
Protective effect of Phyllanthus emblica fruit extract against hydrogen peroxide-induced endothelial cell death

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876837988

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S40-S48

Suggested Citation

Wongpradabchai S., Chularojmontri L., Phornchirasilp S., Wattanapitayakul S.K. Protective effect of Phyllanthus emblica fruit extract against hydrogen peroxide-induced endothelial cell death. Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S40-S48. Retrieved from: https://hdl.handle.net/20.500.14740/6776

Title

Protective effect of Phyllanthus emblica fruit extract against hydrogen peroxide-induced endothelial cell death

Author(s)

Wongpradabchai S.
 Chularojmontri L.
 Phornchirasilp S.
 Wattanapitayakul S.K.

Abstract

Numerous antioxidants from natural products have been shown to lower ROS levels and enhance vascular endothelial function. The fruits of Phyllanthus emblica are well-known in possessing antioxidative properties but its role and mechanisms in the protection of vascular endothelial cells from ROS damage have not yet been established. The present study was aimed to determine the possible protective effect of P. emblica fruit extract (PE) on human EA.hy926 endothelial cell death induced by hydrogen peroxide (H2O2) and PE protective mechanisms. Following incubation of endothelial cells with 300 microM H2O2 for 2 h, cell viability was decreased to 50.65 ± 0.94% and intracellular ROS levels was increased to 159.01% ± 6.27% as measured by MTT assay and DCF fluorescent intensity, respectively. Cytotoxic effect of PE was not observed in the range of 0.1 to 100 microM. Pretreatment with PE (20 to 100 microg/mL) for 48 h significantly ameliorated the cytotoxic effect of H2O2 and attenuated the excessive intracellular ROS formation in endothelial cells. In addition, western blot analysis revealed that PE pretreatment (40 microg/L) induced Akt phosphorylation but did not activate NF-kappaB pathway. These findings suggest that PE could effectively protect human endothelial cell death induced by H2O2 via modification of ROSrelated mechanism along with activation of PI3K/Akt pathway. However, the value of this plant in vivo needs further investigations in supporting them to be developed as nutraceuticals for cardiovascular disease prevention.

Subject(s)

Antioxidant
 Emblica officinalis extract
 Flavonoid
 Hydrogen peroxide
 Immunoglobulin enhancer binding protein
 Protein kinase B
 Reactive oxygen metabolite
 Article
 Cardiovascular disease
 Cell death
 Cell viability
 Colorimetry
 Controlled study
 Cytotoxicity
 Emblica officinalis
 Endothelium cell
 Fluorescence analysis
 Fruit
 Human cell
 Humna
 Oxidative stress
 Prophylaxis
 Protein phosphorylation
 Western blotting
 Analysis of Variance
 Blotting, Western
 Cell Death
 Cells, Cultured
 Endothelial Cells
 Humans
 Hydrogen Peroxide
 NF-kappa B
 Oxidative Stress
 Phosphatidylinositol 3-Kinases
 Phosphorylation
 Phyllanthus emblica
 Plant Extracts
 Reactive Oxygen Species

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URI

https://hdl.handle.net/20.500.14740/6776

Collections

Scopus: Year 1983-2021

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