Publication:
Efficient delivery of antisense oligodeoxyribonucleotide G3139 by human serum albumin-coated liposomes

0
0

Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

15438384

DOI

10.1021/mp900150g

Other identifier(s)

2-s2.0-72049109189

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular Pharmaceutics. Vol 6, No.6 (2009), p.1848-1855

Suggested Citation

Weecharangsan W., Yu B., Zheng Y., Liu S., Pang J.X., Lee L.J., Marcucci G., Lee R.J. Efficient delivery of antisense oligodeoxyribonucleotide G3139 by human serum albumin-coated liposomes. Molecular Pharmaceutics. Vol 6, No.6 (2009), p.1848-1855. doi:10.1021/mp900150g Retrieved from: https://hdl.handle.net/20.500.14740/7695

Title

Efficient delivery of antisense oligodeoxyribonucleotide G3139 by human serum albumin-coated liposomes

Author(s)

Weecharangsan W.
 Yu B.
 Zheng Y.
 Liu S.
 Pang J.X.
 Lee L.J.
 Marcucci G.
 Lee R.J.

Abstract

Human serum albumin (HSA)-coated liposomal formulations were synthesized and evaluated for the delivery of antisense oligodeoxyribonucleotide (ODN) G3139 in KB human oral carcinoma cells. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/α-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with G3139 and coated with HSA were investigated for Bcl-2 downregulating activity. Cellular uptake of HSA-coated liposome-ODN complexes was more efficient than the uncoated liposome-ODN complexes. Treatment of the cells with HSA-coated liposome-ODN complexes resulted in efficient Bcl-2 mRNA downregulation that was approximately 3-fold greater than with uncoated liposomes (p < 0.05) and 6-fold greater than with free ODN. The transfection efficiency of liposome-ODN complexes coated with HSA was dependent on the concentration of HSA used and on the contents of α-helix and β-strand in HSA. HSA-coated liposomes are effective delivery vehicles for antisense ODN. © 2009 American Chemical Society.

Subject(s)

Alpha tocopherol
 Dimethyldioctadecyl ammonium bromide
 Human serum albumin
 Liposome
 Oblimersen
 Phosphatidylcholine
 Protein bcl 2
 Unclassified drug
 Alpha helix
 Article
 Carcinoma cell
 Complex formation
 Controlled study
 Down regulation
 Drug formulation
 Genetic transfection
 Human
 Human cell
 Mouth carcinoma
 Priority journal
 Blotting, Western
 Cell Line, Tumor
 Circular Dichroism
 Electrophoresis, Agar Gel
 Humans
 Liposomes
 Proto-Oncogene Proteins c-bcl-2
 Reverse Transcriptase Polymerase Chain Reaction
 Serum Albumin
 Thionucleotides

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/7695

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By