Publication: Anticancer effects of terrein in breast cancer cells via induction of oxidative stress
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0
Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85074997250
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S152-S158
Suggested Citation
Chawsoun S., Jarintanan F., Jongrungruangchok S., Pongsunk S., Uthaisang-Tanechpongtamb W. Anticancer effects of terrein in breast cancer cells via induction of oxidative stress. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S152-S158. Retrieved from: https://hdl.handle.net/20.500.14740/4372
Abstract
Background: New anticancer drugs that eradicate specifically to cancer cells are needed to develop. In this study, terrein a compound isolated from Aspergillus terreus, was selected to test for the anticancer activity and oxidative-induced cancer cell death as target mechanism. Objective: To determine the cytotoxicity of terrein comparing between breast cancer and non-cancerous cells and measuring the level of reactive oxygen species (ROS) and glutathione (GSH). Material and Method: MTT assay was used to test the cytotoxic level of terrein in MDA-MB-231 (a triple negative breast cancer cell line) comparing to Vero, a non-cancerous cells. For the amount of ROS, cell permeable fluorescent probe DCFDA was used and detected with flow cytometer, while the level of GSH was determined by using spectrophotometer. Results: The result showed that terrein was cytotoxic to MDA-MB-231 at higher level than in Vero cells, the IC50 was at 0.09 and 0.57 mM, respectively. The amount of ROS was increased in a dose-dependent manner, while GSH content was reduced with dose- and time-dependent manner. Conclusion: The possible mechanism to induce breast cancer cell death by terrein is via the induction of oxidative stress and reduction of antioxidant GSH level. Hence, these data support the notion that terrein is an interesting compound to develop as anticancer agent. © 2017 Medical Association of Thailand. All rights reserved.
Subject(s)
Antineoplastic agent
Antioxidant
Glutathione
Reactive oxygen metabolite
Terrein
Animal cell
Antineoplastic activity
Article
Breast cancer
Breast cancer cell line
Cell death
Cell proliferation assay
Chemical structure
Controlled study
Cytotoxicity
Drug dose comparison
Flow cytometry
Human
Human cell
IC50
MTT assay
Nonhuman
Oxidative stress
Spectrophotometry
Antioxidant
Glutathione
Reactive oxygen metabolite
Terrein
Animal cell
Antineoplastic activity
Article
Breast cancer
Breast cancer cell line
Cell death
Cell proliferation assay
Chemical structure
Controlled study
Cytotoxicity
Drug dose comparison
Flow cytometry
Human
Human cell
IC50
MTT assay
Nonhuman
Oxidative stress
Spectrophotometry