Publication:
Role of HMGB1 in proliferation and migration of human gingival and periodontal ligament fibroblasts.

1
0

Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

18804926

DOI

10.2334/josnusd.55.45

Other identifier(s)

2-s2.0-84894419309

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of oral science. Vol 55, No.1 (2013), p.45-50

Suggested Citation

Chitanuwat A., Laosrisin N., Dhanesuan N. Role of HMGB1 in proliferation and migration of human gingival and periodontal ligament fibroblasts.. Journal of oral science. Vol 55, No.1 (2013), p.45-50. doi:10.2334/josnusd.55.45 Retrieved from: https://hdl.handle.net/20.500.14740/6740

Title

Role of HMGB1 in proliferation and migration of human gingival and periodontal ligament fibroblasts.

Author(s)

Chitanuwat A.
 Laosrisin N.
 Dhanesuan N.

Abstract

High mobility group box 1 (HMGB1) was originally defined as a nuclear protein. However, later studies showed that HMGB1 was released from damaged cells into the extracellular milieu and functioned as a danger signaling molecule. HMGB1 has also been shown to exert proliferative and chemoattractant effects on many cell types. In this study, we investigated the in vitro effect of human recombinant HMGB1 on the proliferation and migration of human gingival fibroblasts (HGF) and human periodontal ligament fibroblasts (HPDLF). For the proliferation assay, HGF and HPDLF were cultured in the presence of 5, 10, and 50 ng/mL HMGB1. After a period of 6 days, cell proliferation was determined by MTT assay. The migration assay was performed by culturing the two cell types in Transwells with HMGB1 in the lower chamber as a chemoattractant. Cell migration during 16 h was determined by crystal violet staining of the cells that migrated across the membrane. The results showed that HMGB1, at 50 ng/mL, was able to significantly induce proliferation of HGF by up to 171.4 ± 17.1%. No such proliferation induction was seen for HPDLF. In the migration assay, however, 100 ng/mL HMGB1 induced migration of both cell types. The counts of cells that migrated across the membrane, as compared with the control, were increased to 273 ± 24.1% and 410.3 ± 158% for HGF and HPDLF, respectively. Since proliferation and migration are basic abilities of cells required for proper tissue repair, these data suggest that HMGB1 plays an important role in these functions of periodontal cells.

Subject(s)

Chemotactic factor
 Coloring agent
 Crystal violet
 High mobility group B1 protein
 HMGB1 protein, human
 Recombinant protein
 Tetrazolium
 Thiazole derivative
 Thiazolyl blue
 Cell count
 Cell motion
 Cell proliferation
 Chemotaxis
 Comparative study
 Culture technique
 Cytology
 Diagnostic use
 Drug effects
 Fibroblast
 Gingiva
 Human
 Periodontal ligament
 Time
 Cell Count
 Cell Culture Techniques
 Cell Movement
 Cell Proliferation
 Chemotactic Factors
 Chemotaxis
 Coloring Agents
 Fibroblasts
 Gentian Violet
 Gingiva
 HMGB1 Protein
 Humans
 Periodontal Ligament
 Recombinant Proteins
 Tetrazolium Salts
 Thiazoles
 Time Factors

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6740

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By