Publication:
Effect of incubation temperature on lactogenic function of goat milk-derived mammary epithelial cells

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

10712690

DOI

10.1007/s11626-020-00529-3

Other identifier(s)

2-s2.0-85096050785

Rights

Srinakharinwirot University

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Scopus

Bibliographic Citation

In Vitro Cellular and Developmental Biology - Animal. Vol 56, No.10 (2020), p.842-846

Suggested Citation

Saipin N., Thuwanut P., Thammacharoen S., Rungsiwiwut R. Effect of incubation temperature on lactogenic function of goat milk-derived mammary epithelial cells. In Vitro Cellular and Developmental Biology - Animal. Vol 56, No.10 (2020), p.842-846. doi:10.1007/s11626-020-00529-3 Retrieved from: https://hdl.handle.net/20.500.14740/5213

Title

Effect of incubation temperature on lactogenic function of goat milk-derived mammary epithelial cells

Author(s)

Saipin N.
 Thuwanut P.
 Thammacharoen S.
 Rungsiwiwut R.

Abstract

In general, goat mammary epithelial cells (MECs) are cultured in vitro under 37 °C. We demonstrated previously that goat MECs differentiate under 37 °C although their body temperature is approximately 39 °C. This study aimed to investigate the influence of 39 °C on lactogenic differentiation of goat milk-derived MECs. The results revealed that HSP70 gene was significantly elevated at 1 h after an exposure to 39 °C but declined at 48 h thereafter. Oxidative stress status was not significantly affected by 39 °C. Expressions of CSN2, β-GALT1, α-LA, and Akt genes tended to increase after the differentiation under 39 °C. Secretion of lactose under 39 °C was not significantly lower than 37 °C. In conclusion, incubation temperature at 39 °C does not dramatically affect lactogenic function of goat milk-derived MECs. © 2020, The Society for In Vitro Biology.

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URI

https://hdl.handle.net/20.500.14740/5213

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Scopus: Year 1983-2021

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