Publication:
Ultraviolet B irradiation-induced keratinocyte senescence and impaired development of 3D epidermal reconstruct

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Issued Date

2021

Resource Type

Article

File Type

application/pdf

ISSN

13300075

DOI

10.2478/acph-2021-0011

Other identifier(s)

2-s2.0-85095862250

Rights

Srinakharinwirot University

Rights Holder(s)

Scopus

Bibliographic Citation

Acta Pharmaceutica. Vol 71, No.2 (2021), p.293-303

Suggested Citation

Wattanapitayakula S.K., Chularojmontri L., Schäfer-Korting M. Ultraviolet B irradiation-induced keratinocyte senescence and impaired development of 3D epidermal reconstruct. Acta Pharmaceutica. Vol 71, No.2 (2021), p.293-303. doi:10.2478/acph-2021-0011 Retrieved from: https://hdl.handle.net/20.500.14740/4507

Title

Ultraviolet B irradiation-induced keratinocyte senescence and impaired development of 3D epidermal reconstruct

Author(s)

Wattanapitayakula S.K.
 Chularojmontri L.
 Schäfer-Korting M.

Abstract

Ultraviolet B (UVB) induces morphological and functional changes of the skin. This study investigated the effect of UVB on keratinocyte senescence and the development of reconstructed human epidermis (RHE). Primary normal human keratinocytes (NHK) from juvenile foreskin were irradiated with UVB (30 mJ cm–2) and these effects were compared to NHK that underwent senescence in the late passage. UVB enhanced the accumulation of reactive oxygen species (ROS) and halted cell replication as detected by BrdU cell proliferation assay. The senescence phenotype was evaluated by beta-galactosidase (β-gal) staining and qPCR of genes related to senescent regulation, i.e. p16INK4a, cyclin D2, and IFI27. Senescence induced by high dose UVB resulted in morphological changes, enhanced β-gal activity, elevated cellular ROS levels and reduced DNA synthesis. qPCR revealed differential expression of the genes regulated senescence. p16INK4a expression was significantly increased in NHK exposed to UVB whereas enhanced IFI27 expression was observed only in cultural senescence. The levels of cyclin D2 expression were not significantly altered either by UVB or long culturing conditions. UVB significantly induced the aging phenotype in keratinocytes and impaired epidermal development. RHE generated from UVB-irradiated keratinocytes showed a thinner cross-sectional structure and the majority of keratinocytes in the lower epidermis were degenerated. The 3D epidermis model is useful in studying the skin aging process. © 2021 Sciendo. All rights reserved.

Subject(s)

Epidermis
 Keratinocytes
 Reactive oxygen species
 Senescence
 Skin aging
 Ultraviolet radiation
 Beta galactosidase
 Cyclin D2
 Cyclin dependent kinase inhibitor 2A
 Hydrogen peroxide
 Hydroxyl radical
 Interleukin 1beta
 Interleukin 6
 Mammalian target of rapamycin
 Peroxynitrite
 Reactive oxygen metabolite
 Superoxide
 Aging
 Article
 BrdU assay
 Cell division
 Cell isolation
 Cell proliferation
 Cell proliferation assay
 Cell survival
 Cell viability
 Controlled study
 Cutaneous parameters
 DNA damage
 DNA microarray
 DNA repair
 Epidermis
 Flow cytometry
 Genomic instability
 Histology
 Human
 Human cell
 Keratinocyte
 Microscopy
 Oxidative stress
 Phenotype
 Photoaging
 Protein expression
 Radiation exposure
 Reverse transcription polymerase chain reaction
 Senescence
 Ultraviolet B radiation

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URI

https://hdl.handle.net/20.500.14740/4507

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Scopus: Year 1983-2021

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