Publication:
Sox9 is upstream of microRNA-140 in cartilage

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

2732289

DOI

10.1007/s12010-011-9404-y

Other identifier(s)

2-s2.0-84856260583

Rights Holder(s)

Scopus

Bibliographic Citation

Applied Biochemistry and Biotechnology. Vol 166, No.1 (2012), p.64-71

Suggested Citation

Nakamura Y., He X., Kato H., Wakitani S., Kobayashi T., Watanabe S., Iida A., Tahara H., Warman M.L., Watanapokasin R., Postlethwait J.H. Sox9 is upstream of microRNA-140 in cartilage. Applied Biochemistry and Biotechnology. Vol 166, No.1 (2012), p.64-71. doi:10.1007/s12010-011-9404-y Retrieved from: https://hdl.handle.net/20.500.14740/7144

Title

Sox9 is upstream of microRNA-140 in cartilage

Author(s)

Nakamura Y.
 He X.
 Kato H.
 Wakitani S.
 Kobayashi T.
 Watanabe S.
 Iida A.
 Tahara H.
 Warman M.L.
 Watanapokasin R.
 Postlethwait J.H.

Abstract

MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a-/-) and sox9b mutant (sox9b-/-) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells. © 2011 Springer Science+Business Media, LLC.

Subject(s)

In situ hybridization
 MicroRNA-140 (miR-140)
 RT-PCR
 SiRNA
 Sox9
 T/C-28
 Zebrafish
 Mammals
 Polymerase chain reaction
 RNA
 Tissue
 Cartilage
 MicroRNA
 MicroRNA 140
 Mutant protein
 Platelet derived growth factor alpha receptor
 Small interfering RNA
 Transcription factor Sox9
 Unclassified drug
 Animal experiment
 Article
 Cartilage
 Cartilage cell
 Controlled study
 Human
 Human cell
 In situ hybridization
 Nonhuman
 Protein expression
 Reverse transcription polymerase chain reaction
 Transcription regulation
 Zebra fish
 Animals
 Binding Sites
 Cartilage
 Chondrocytes
 Chondrogenesis
 Embryonic Development
 Gene Expression Regulation, Developmental
 Humans
 In Situ Hybridization
 MicroRNAs
 Phylogeny
 Protein Binding
 RNA, Small Interfering
 SOX9 Transcription Factor
 Ubiquitin-Protein Ligases
 Zebrafish
 Danio rerio
 Mammalia

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URI

https://hdl.handle.net/20.500.14740/7144

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Scopus: Year 1983-2021

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