Publication: Protective effect of α-mangostin against type-I collagen formation in thioacetamide-induced cirrhotic rat
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Issued Date
2012
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-84876906258
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S93-S98
Suggested Citation
Poonkhum R., Watanapokasin R., Pradidarcheep W. Protective effect of α-mangostin against type-I collagen formation in thioacetamide-induced cirrhotic rat. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S93-S98. Retrieved from: https://hdl.handle.net/20.500.14740/6877
Author(s)
Abstract
Objective: To elucidate the protective effect of α-mangostin (α-MG) against increment of type-I collagen-positive hepatocytes in rat cirrhosis induced by thioacetamide (TAA). Material and Method: Rats were separated into 4 groups. The first group was, the control, untreated with TAA. The cirrhotic rats, the second group, were induced by TAA injection (200 mg/kg), 3 times per week. Rats in the third group received treatment of TAA (200 mg/kg) alternating with α-MG (100 mg/kg) for every other day. Animals in the last group were treated only with α-MG (100 mg/kg), 3 times per week. The chemicals used each group were given intraperitoneally for 16 weeks. The type-I collagen and type-I collagen-positive hepatocytes were explored by using immunohistochemical technique. Results: In cirrhotic livers type-I collagen was immunopositive in the connective tissue and a large number of hepatocytes. The number of type I collagen-positive-hepatocytes (414.00 ± 25.23) in TAA-induced cirrhosis group increased significantly when compared to those in the control group (131.40 ± 9.63). Interestingly, a significant decrease in the number of type-I collagen-positive-hepatocytes was observed in TAA-α-MG-prevention group (103.60 ± 36.55) and in α-MG-injected group (54.00 ± 5.30) compared to those in the control group and TAA-induced cirrhosis. Conclusion: 100 mg/kg of α-MG could lower the number of type-I collagen-positive-hepatocytes in TAA-induced cirrhosis. It is probable that α-MG helps to keep up more blood circulation to the liver cells through dilated sinusoids. This vascular adaptation enhances high oxygen blood to the hepatocytes which, in turn, reduces the damage of hepatocytes caused by TAAderived reactive oxygen species.
Subject(s)
Alpha mangostin
Collagen fiber
Collagen type 1
Thioacetamide
Unclassified drug
Xanthone derivative
Animal experiment
Animal model
Animal tissue
Article
Cell protection
Controlled study
Immunohistochemistry
Liver cell
Liver cirrhosis
Liver sinusoid
Male
Nonhuman
Rat
Analysis of Variance
Animals
Collagen Type I
Hepatocytes
Liver Cirrhosis, Experimental
Male
Phytotherapy
Rats
Rats, Wistar
Thioacetamide
Xanthones
Collagen fiber
Collagen type 1
Thioacetamide
Unclassified drug
Xanthone derivative
Animal experiment
Animal model
Animal tissue
Article
Cell protection
Controlled study
Immunohistochemistry
Liver cell
Liver cirrhosis
Liver sinusoid
Male
Nonhuman
Rat
Analysis of Variance
Animals
Collagen Type I
Hepatocytes
Liver Cirrhosis, Experimental
Male
Phytotherapy
Rats
Rats, Wistar
Thioacetamide
Xanthones