Publication: Identification of species in the Cladia aggregata group using DNA barcoding (Ascomycota: Lecanorales)
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
11793155
Other identifier(s)
2-s2.0-84879656085
Rights Holder(s)
Scopus
Bibliographic Citation
Phytotaxa. Vol 115, No.1 (2013), p.1-14
Suggested Citation
Parnmen S., Leavitt S.D., Rangsiruji A., Lumbsch H.T. Identification of species in the Cladia aggregata group using DNA barcoding (Ascomycota: Lecanorales). Phytotaxa. Vol 115, No.1 (2013), p.1-14. doi:10.11646/phytotaxa.115.1.1 Retrieved from: https://hdl.handle.net/20.500.14740/6634
Author(s)
Abstract
The DNA barcode approach is using a short genetic marker for rapid identification of a particular species. The internal transcribed spacer (ITS) region has been chosen as barcode marker for fungi. Here we tested the potential of ITS to identify distinct lineages in the Cladia aggregata complex, a group of lichenized fungi exhibiting remarkable morphological and chemical diversity. Our recent studies using multilocus DNA sequence data and coalescent-based species delimitation methods supported a 12 species delimitation scenario. In this study, we evaluated the ratio of the intra- and interspecific genetic distances of ITS in these 12 putative species. All 12 putative species showed a lower ratio of intraspecific variation than interspecific variation, supporting the hypothesis that these represent distinct lineages. Consequently, these lineages are here accepted at species level and three new species, viz. Cladia blanchonii Parnmen & Lumbsch, C. cryptica Parnmen & Lumbsch and C. tasmanica Parnmen & Lumbsch are described and the new combinations Cladia gorgonea (Eschw.) Parnmen & Lumbsch, C. neocaledonica (Räs.) Parnmen & Lumbsch, and C. terebrata (Laurer) Parnmen & Lumbsch proposed. © 2013 Magnolia Press.