Publication: Lipid production from sugarcane top hydrolysate and crude glycerol with rhodosporidiobolus fluvialis using a two-stage batch-cultivation strategy with separate optimization of each stage
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Issued Date
2020
Resource Type
File Type
application/pdf
ISSN
20762607
Other identifier(s)
2-s2.0-85083649181
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Microorganisms. Vol 8, No.3 (2020)
Suggested Citation
Boonyarit J., Polburee P., Khaenda B., Zhao Z.K., Limtong S. Lipid production from sugarcane top hydrolysate and crude glycerol with rhodosporidiobolus fluvialis using a two-stage batch-cultivation strategy with separate optimization of each stage. Microorganisms. Vol 8, No.3 (2020). doi:10.3390/microorganisms8030453 Retrieved from: https://hdl.handle.net/20.500.14740/4646
Author(s)
Abstract
�Lipids from oleaginous microorganisms, including oleaginous yeasts, are recognized as feedstock for biodiesel production. A production process development of these organisms is necessary to bring lipid feedstock production up to the industrial scale. This study aimed to enhance lipid production of low-cost substrates, namely sugarcane top and biodiesel-derived crude glycerol, by using a two-stage cultivation process with Rhodosporidiobolus fluvialis DMKU-SP314. In the first stage, sugarcane top hydrolysate was used for cell propagation, and in the second stage, cells were suspended in a crude glycerol solution for lipid production. Optimization for high cell mass production in the first stage, and for high lipid production in the second stage, were performed separately using a one-factor-at-a-time methodology together with response surface methodology. Under optimum conditions in the first stage (sugarcane top hydrolysate broth containing; 43.18 g/L total reducing sugars, 2.58 g/L soy bean powder, 0.94 g/L (NH4)2SO4, 0.39 g/L KH2PO4 and 2.5 g/L MgSO4•7H2O, pH 6, 200 rpm, 28 ∘C and 48 h) and second stage (81.54 g/L crude glycerol, pH 5, 180 rpm, 27 ∘C and 196 h), a high lipid concentration of 15.85 g/L, a high cell mass of 21.07 g/L and a high lipid content of 73.04% dry cell mass were obtained. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.