Publication: Inhibitory Effect of Inorganic Salts Residuals on Cellulase Kinetics in Biofuel Production from Lignocellulose Biomass
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Issued Date
2020
Resource Type
File Type
application/pdf
Other identifier(s)
2-s2.0-85100057006
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Proceedings of the 2020 International Conference and Utility Exhibition on Energy, Environment and Climate Change, ICUE 2020. (2020)
Suggested Citation
Mutrakulcharoen P., Pornwongthong P., Cheenkachorn K., Tantayotai P., Roddecha S., Sriarivanun M. Inhibitory Effect of Inorganic Salts Residuals on Cellulase Kinetics in Biofuel Production from Lignocellulose Biomass. Proceedings of the 2020 International Conference and Utility Exhibition on Energy, Environment and Climate Change, ICUE 2020. (2020). doi:10.1109/ICUE49301.2020.9307055 Retrieved from: https://hdl.handle.net/20.500.14740/4341
Abstract
Biorefining process has the goal to convert lignocellulose biomass to biofuels and value-added products and to reduce burning activities of agricultural wastes after harvesting seasons. Pretreatment of lignocellulose biomass by using inorganic salt has been demonstrated to be an efficient method to improve process productivity, yet its efficiency is inhibited by salt residues in hydrolysis. In this study, the mechanisms of inhibitory effects caused by inorganic salts, NaCl and KCl, on kinetic activities of commercial cellulase were revealed using Michaelis-Menten model. Three different types of cellulose and lignocellulose substrates, CMC, Avicel, and sugarcanes bagasse, were enzymatic hydrolyzed. Using Lineweaver-Burk plot, the results showed that the inhibitory effect of KCl and NaCl residues were non-competitive and mixed inhibition, respectively. Due to their inhibitory effect, inorganic salt residues reduced reducing sugar yields released from CMC and Avicel, however, the yield obtained from sugarcanes bagasse increased for 1.51 fold when adding 1.0 M NaCl. The finding in this research suggested the alternative method to use sugarcane bagasse for biofuel conversion and reduction of agricultural combustion. © 2020 IEEE.
Subject(s)
Agricultural robots
Agricultural wastes
Agriculture
Bagasse
Biofuels
Biomass
Cellulose
Cellulose derivatives
Climate change
Hydrolysis
Lignin
Sodium chloride
Waste incineration
Biofuel production
Cellulase kinetics
Lineweaver-Burk plots
Michaelis-Menten model
Mixed inhibitions
Process productivity
Sugar-cane bagasse
Value added products
Potassium compounds
Agriculture
Bagasse
Biomass
Cellulose Derivatives
Hydrolysis
Agricultural wastes
Agriculture
Bagasse
Biofuels
Biomass
Cellulose
Cellulose derivatives
Climate change
Hydrolysis
Lignin
Sodium chloride
Waste incineration
Biofuel production
Cellulase kinetics
Lineweaver-Burk plots
Michaelis-Menten model
Mixed inhibitions
Process productivity
Sugar-cane bagasse
Value added products
Potassium compounds
Agriculture
Bagasse
Biomass
Cellulose Derivatives
Hydrolysis