Publication:
Antioxidant effects of gamma-oryzanol on human prostate cancer cells

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

15137368

DOI

10.7314/APJCP.2013.14.9.5421

Other identifier(s)

2-s2.0-84887568262

Rights Holder(s)

Scopus

Bibliographic Citation

Asian Pacific Journal of Cancer Prevention. Vol 14, No.9 (2013), p.5421-5425

Suggested Citation

Klongpityapong P., Supabphol R., Supabphol A. Antioxidant effects of gamma-oryzanol on human prostate cancer cells. Asian Pacific Journal of Cancer Prevention. Vol 14, No.9 (2013), p.5421-5425. doi:10.7314/APJCP.2013.14.9.5421 Retrieved from: https://hdl.handle.net/20.500.14740/6749

Title

Antioxidant effects of gamma-oryzanol on human prostate cancer cells

Author(s)

Klongpityapong P.
 Supabphol R.
 Supabphol A.

Abstract

Background: To assess the antioxidant effects of gamma-oryzanol on human prostate cancer cells. Materials and Methods: Cytotoxic activity of gamma-oryzanol on human DU145 and PC3 prostate cancer cells was determined by proliferation assay using 3-(4, 5-dimethylthiazol, 2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent. mRNA levels of genes involved in the intracellular antioxidant system, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GSR) were determined by reverse transcription-polymerase chain reaction (RT-PCR). Cancer cell lysates were used to measure lipid peroxidation using thiobarbituric acid reactive substance (TBARS). Glutathione contents of the cell lysates were estimated by the reaction between sulfhydryl group of 5, 5'-dithio (bis) nitrobenzoic acid (DTNB) to produce a yellow-color of 5-thio-2-nitrobenzoic acid using colorimetric assay. Catalase activity was also analysed by examining peroxidative function. Protein concentration was estimated by Bradford's assay. Results: All concentrations of gamma-oryzanol, 0.1-2.0mg/ml, significantly inhibited cell growth in a dose- and time-dependent fashion in both prostate cancer cell lines, DU145 and PC3. Gene expression of catalase in DU145 and PC3 exposed to gamma-orizanol at 0.5mg/ml for 14 days was down regulated, while mRNA of GPX was also down regulated in PC3. The MDA and glutathione levels including catalase activity in the cell lysates of DU145 and PC3 treated with gamma-oryzanol 0.1 and 0.5mg/ml were generally decreased. Conclusions: This study highlighted effects of gamma-oryzanol via the down-regulation of antioxidant genes, catalase and GPX, not cytotoxic roles. This might be interesting for adjuvant chemotherapy to make prostate cancer cells more sensitive to free radicals. It might be useful for the reduction of cytotoxic agents and cancer chemoprevention.

Subject(s)

Antilipemic agent
 Antioxidant
 Catalase
 Gamma oryzanol
 Gamma-oryzanol
 Glutathione
 Malonaldehyde
 Messenger RNA
 Phenylpropionic acid derivative
 Apoptosis
 Article
 Cell proliferation
 Drug effect
 Enzyme linked immunosorbent assay
 Genetics
 Human
 Lipid peroxidation
 Male
 Metabolism
 Pathology
 Prostate tumor
 Real time polymerase chain reaction
 Reverse transcription polymerase chain reaction
 Tumor cell culture
 Western blotting
 Antioxidants
 Apoptosis
 Blotting, Western
 Catalase
 Cell Proliferation
 Enzyme-Linked Immunosorbent Assay
 Glutathione
 Humans
 Hypolipidemic Agents
 Lipid Peroxidation
 Male
 Malondialdehyde
 Phenylpropionates
 Prostatic Neoplasms
 Real-Time Polymerase Chain Reaction
 Reverse Transcriptase Polymerase Chain Reaction
 RNA, Messenger
 Tumor Cells, Cultured

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URI

https://hdl.handle.net/20.500.14740/6749

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Scopus: Year 1983-2021

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